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Western blot analysis of Phospho-NMDA NR2B Subunit pTyr1472 in rat hippocampal lysate using a polyclonal antibody (Product # 38-7000). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1472 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
|Tested species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to amino acid residues surrounding the phosphorylated Tyr1472 of rat NMDA receptor, NR2B subunit.|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
GluN2B; Glutamate [NMDA] receptor subunit epsilon 2 precursor; Glutamate [NMDA] receptor subunit epsilon-2; Glutamate receptor ionotropic, NMDA 2B; glutamate receptor, ionotropic, NMDA2B; GRIN2B; hNR3; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subtype 2B; NMDAR2B; NMDE2; NME2; NR2B