Western blot analysis of Phospho-NPM pThr234 using Phospho-NPM pThr234 polyclonal antibody (Product # PA5-36786) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with colchicine (0.2ug/ml, 24h), Lane 2: Raw264.7 cells treated with colchicine (0.2ug/ml, 24h), Lane 3: PC12 cell lysate treated with colchicine (0.2ug/ml, 24h).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human NPM around the phosphorylation site of Threonine 234|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 38 kDa.
Purity is >95% by SDS-PAGE.
Nucleophosmin (NPM; also known as B23, numatrin or NO38) is an abundant phosphoprotein primarily found in nucleoli. It has been implicated in several distinct cellular functions, including assembly and transport of ribosomes, cytoplasmic/nuclear trafficking, regulation of DNA polymerase alpha activity, centrosome duplication and molecular chaperoning activities. The NPM gene is also known for its fusion with the anaplastic lymphoma kinase (ALK) receptor tyrosine kinase. The NPM portion contributes to transformation by providing a dimerization domain, which results in activation of the fused kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.