Immunofluorescent analysis of SY5Y cells using a Phospho-Nanog pSer71 polyclonal antibody (Product # PA5-13078). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with a Phospho-Nanog pSer71 polyclonal antibody (Product # PA5-13078) (1:500, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Cytoplasmic actin was counterstained with red dye conjugated phalloidin (5.25 uM, 25 min).
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S71 of human Nanog|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with non-human primate based on sequence homology.
NANOG is a Ttranscription regulator involved in inner cell mass and embryonic stem (ES) cels proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.