|Tested species reactivity||Bovine, Chicken, Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Native NF-H purified from bovine spinal cord|
|Storage buffer||PBS with 50% glycerol|
|Contains||5mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||1:10000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
This antibody recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences.
Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the neurofilament high or heavy molecular weight polypeptide and runs on SDS-PAGE gels at 200-220 kDA, with some variability across species boundaries. Antibodies to NF-H are useful for identifying neuronal cells and their proceses in tissue sections and in tissue culture. NF-H antibodies can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Amyothrophic lateral sclerosis (also known as Lou Gehrig's disease) and Alzheimer's disease. NAP4 is one of numerous antibodies which reacts preferentially with the phosphorylated forms of NF-H. These forms have an apparent sized determined from SDS-PAGE gels at about 40 kDs greater than the non-phosphorylated forms. Interestingly the phosphorylated forms of NF-H are normally restricted to axons, while less phosphorylated forms are found in dendrites. However in numerous damage and disease states, phosphorylated NF-H can be detected in NAP4 in dendritic and perikaryal neurofilaments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Compartmentation of alpha-internexin and neurofilament triplet proteins in cultured hippocampal neurons.
MA1-10041 was used in immunocytochemistry and western blot to study the subcellular localization of alpha internexin and neurofilament triplet proteins
|Benson DL,Mandell JW,Shaw G,Banker G||Journal of neurocytology (25:181)||1996|