|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S1834 of human P300|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Dot blot (DB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CBP (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CBP can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
G Protein-coupled Receptor 40 (GPR40) and Peroxisome Proliferator-activated Receptor γ (PPARγ): AN INTEGRATED TWO-RECEPTOR SIGNALING PATHWAY.
PA5-12735 was used in western blot to investigate GPR40 and PPARgamma signaling interactions in endothelial cells
|Wang S,Awad KS,Elinoff JM,Dougherty EJ,Ferreyra GA,Wang JY,Cai R,Sun J,Ptasinska A,Danner RL||The Journal of biological chemistry (290:19544)||2015|
Laminar shear stress upregulates endothelial Ca²⁺-activated K⁺ channels KCa2.3 and KCa3.1 via a Ca²⁺/calmodulin-dependent protein kinase kinase/Akt/p300 cascade.
PA5-12735 was used in western blot to study the role of a cascade involving Ca(2+), CaMKK, Akt and p300 in the upregulation of the endothelial expression of KCa2.3 and KCa3.1 by laminar shear stress
|Takai J,Santu A,Zheng H,Koh SD,Ohta M,Filimban LM,Lemaître V,Teraoka R,Jo H,Miura H||American journal of physiology. Heart and circulatory physiology (305:H484)||2013|