Immunofluorescence analysis of Phospho-P38 pThr180 / pTyr182 Antibody was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Phospho-P38 pThr180 / pTyr182 Antibody (44684g) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa flour 488 Goat Anti-Rabbit IgG Secondary Antibody (A11008) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (A12381). Panel d is a merged image showing nuclear localization. Panel e is a no primary antibody control. The images were captured at 40X magnification.
|Tested species reactivity||Human, Rat|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including mouse, rat, dog, monkey and carp.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/million cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 16 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases, or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cathepsin S attenuates endosomal EGFR signalling: A mechanical rationale for the combination of cathepsin S and EGFR tyrosine kinase inhibitors.
44-684G was used in western blot to characterize attenuation of endosomal EGFR signaling by cathepsin S as a mechanical rationale for the combination of EGFR tyrosine kinase and cathepsin S inhibitors
|Huang CC,Lee CC,Lin HH,Chang JY||Scientific reports (6:null)||2016|
|Not Applicable||Not Cited||
MAPK14/p38¿-dependent modulation of glucose metabolism affects ROS levels and autophagy during starvation.
44-684G was used in immunocytochemistry and western blot to investigate MAPK14-driven metabolic reprogramming
|Desideri E,Vegliante R,Cardaci S,Nepravishta R,Paci M,Ciriolo MR||Autophagy (10:1652)||2014|
The genomic landscape of diffuse intrinsic pontine glioma and pediatric non-brainstem high-grade glioma.
44-684G was used in western blot to identify signaling pathways that contribute to pediatric high-grade glioma
|Wu G,Diaz AK,Paugh BS,Rankin SL,Ju B,Li Y,Zhu X,Qu C,Chen X,Zhang J,Easton J,Edmonson M,Ma X,Lu C,Nagahawatte P,Hedlund E,Rusch M,Pounds S,Lin T,Onar-Thomas A,Huether R,Kriwacki R,Parker M,Gupta P,Becksfort J,Wei L,Mulder HL,Boggs K,Vadodaria B,Yergeau D,Russell JC,Ochoa K,Fulton RS,Fulton LL,Jones C,Boop FA,Broniscer A,Wetmore C,Gajjar A,Ding L,Mardis ER,Wilson RK,Taylor MR,Downing JR,Ellison DW,Zhang J,Baker SJ||Nature genetics (46:444)||2014|
Expansion of oligodendrocyte progenitor cells following SIRT1 inactivation in the adult brain.
44-684G was used in western blot to elucidate factors that regulate the generation of adult oligodendrocytes.
|Rafalski VA,Ho PP,Brett JO,Ucar D,Dugas JC,Pollina EA,Chow LM,Ibrahim A,Baker SJ,Barres BA,Steinman L,Brunet A||Nature cell biology (15:614)||2013|
|Human||Not Cited||Molecular pathway profiling of T lymphocyte signal transduction pathways; Th1 and Th2 genomic fingerprints are defined by TCR and CD28-mediated signaling.||Smeets RL,Fleuren WW,He X,Vink PM,Wijnands F,Gorecka M,Klop H,Bauerschmidt S,Garritsen A,Koenen HJ,Joosten I,Boots AM,Alkema W||BMC immunology (13:null)||2012|
|Mouse||Not Cited||Growth-factor receptor-bound protein-2 (Grb2) signaling in B cells controls lymphoid follicle organization and germinal center reaction.||Jang IK,Cronshaw DG,Xie LK,Fang G,Zhang J,Oh H,Fu YX,Gu H,Zou Y||Proceedings of the National Academy of Sciences of the United States of America (108:7926)||2011|
|Human||Not Cited||T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.||Adachi K,Davis MM||Proceedings of the National Academy of Sciences of the United States of America (108:1549)||2011|
TGFbeta1 antagonistic peptides inhibit TGFbeta1-dependent angiogenesis.
44-684G was used in western blot to investigate the cancer blocking activity of TGFbeta antagonist peptides
|Serratì S,Margheri F,Pucci M,Cantelmo AR,Cammarota R,Dotor J,Borràs-Cuesta F,Fibbi G,Albini A,Del Rosso M||Biochemical pharmacology (77:813)||2009|
|Not Applicable||Not Cited||
Chemotherapeutic agents up-regulate the cytomegalovirus promoter: implications for bioluminescence imaging of tumor response to therapy.
44-684G was used in western blot to study the promoter-dependent increase in bioluminescence intensity from CMV-driven luciferase
|Svensson RU,Barnes JM,Rokhlin OW,Cohen MB,Henry MD||Cancer research (67:10445)||2007|
|Mouse||Not Cited||Critical role of c-jun (NH2) terminal kinase in paracetamol- induced acute liver failure.||Henderson NC,Pollock KJ,Frew J,Mackinnon AC,Flavell RA,Davis RJ,Sethi T,Simpson KJ||Gut (56:982)||2007|
|Human||Not Cited||Molecular mechanisms involved in interleukin-4-induced human neutrophils: expression and regulation of suppressor of cytokine signaling.||Ratthé C,Pelletier M,Chiasson S,Girard D||Journal of leukocyte biology (81:1287)||2007|
|Human||Not Cited||The mitogen-activated protein kinases (MAPK) p38 and JNK are markers of tumor progression in breast carcinoma.||Davidson B,Konstantinovsky S,Kleinberg L,Nguyen MT,Bassarova A,Kvalheim G,Nesland JM,Reich R||Gynecologic oncology (102:453)||2006|
Chemokine receptor CCR2 expression by systemic sclerosis fibroblasts: evidence for autocrine regulation of myofibroblast differentiation.
44-684G was used in western blot to assess the contribution of CCL2/CCR2 signaling to systemic sclerosis
|Carulli MT,Ong VH,Ponticos M,Shiwen X,Abraham DJ,Black CM,Denton CP||Arthritis and rheumatism (52:3772)||2005|
|Human||Not Cited||TNF-alpha promotes a stop signal that inhibits neutrophil polarization and migration via a p38 MAPK pathway.||Lokuta MA,Huttenlocher A||Journal of leukocyte biology (78:210)||2005|
|Mouse||Not Cited||Urokinase-induced smooth muscle cell responses require distinct signaling pathways: a role for the epidermal growth factor receptor.||Nicholl SM,Roztocil E,Davies MG||Journal of vascular surgery (41:672)||2005|
|Human||Not Cited||Protein synthesis persists during necrotic cell death.||Saelens X,Festjens N,Parthoens E,Vanoverberghe I,Kalai M,van Kuppeveld F,Vandenabeele P||The Journal of cell biology (168:545)||2005|
||T-cell receptor ligation induces distinct signaling pathways in naive vs. antigen-experienced T cells.||Adachi K,Davis MM||Proceedings of the National Academy of Sciences of the United States of America (108:1549)||2011|
Differential modulation of TLR3- and TLR4-mediated dendritic cell maturation and function by progesterone.
44-684G was used in western blot to study how progesterone and TLR agonists affect dendritic cell function
|Jones LA,Kreem S,Shweash M,Paul A,Alexander J,Roberts CW||Journal of immunology (Baltimore, Md. : 1950) (185:4525)||2010|
The peroxisome proliferator-activated receptor gamma agonist pioglitazone reduces the development of cartilage lesions in an experimental dog model of osteoarthritis: in vivo protective effects mediated through the inhibition of key signaling and catabolic pathways.
44-684G was used in immunohistochemistry - paraffin section to assess the effects of pioglitazone treatment on the development of lesions in a canine model of osteoarthritis
|Boileau C,Martel-Pelletier J,Fahmi H,Mineau F,Boily M,Pelletier JP||Arthritis and rheumatism (56:2288)||2007|
||Critical role of c-jun (NH2) terminal kinase in paracetamol- induced acute liver failure.||Henderson NC,Pollock KJ,Frew J,Mackinnon AC,Flavell RA,Davis RJ,Sethi T,Simpson KJ||Gut (56:982)||2007|