Invitrogen

Phospho-PAK1/PAK2 (Thr423, Thr402) Polyclonal Antibody

View all (5) PAK1/PAK2 antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-36888

Applications
Tested Dilution
Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of Threonine 423
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553802

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 60-70 kDa.^M
^M
Purity is >95% by SDS-PAGE.

Target Information

Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: activated protein kinase alpha; Alpha-PAK; CDC42/RAC effector kinase PAK-A; gamma pak; Gamma-PAK; OPHN3; p21 (CDKN1A)-activated kinase 1; p21 (CDKN1A)-activated kinase 2; p21 protein (Cdc42/Rac)-activated kinase 1; p21 protein (Cdc42/Rac)-activated kinase 2; p21-activated kinase 1; p21-activated kinase 2; p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast); p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related); p58; p65-PAK; p68-PAK; PAK alpha; PAK beta; PAK gamma; PAK-1; PAK-2; PAK3; PAK65; Protein kinase MUK2; S6/H4 kinase; Serine/threonine-protein kinase PAK 1; Serine/threonine-protein kinase PAK 2; STE20 homolog, yeast

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Gene Aliases: 5330420P17Rik; A130002K10Rik; AI836325; AW045634; D16Ertd269e; gamma-PAK; mKIAA4182; PAK-1; PAK-2; PAK1; PAK2; PAK65; Paka; PAKalpha; PAKgamma

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UniProt ID: (Human) Q13153, (Human) Q13177, (Rat) P35465, (Mouse) Q8CIN4, (Rat) Q64303

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Entrez Gene ID: (Human) 5058, (Human) 5062, (Mouse) 18479, (Rat) 29431, (Mouse) 224105, (Rat) 29432

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Function(s)
protein kinase activity protein serine/threonine kinase activity receptor signaling protein serine/threonine kinase activity protein binding collagen binding ATP binding protein kinase binding protein tyrosine kinase activator activity identical protein binding cadherin binding involved in cell-cell adhesion nucleotide binding catalytic activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups Rac GTPase binding MAP kinase kinase kinase activity non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade mitotic cell cycle response to hypoxia positive regulation of protein phosphorylation stimulatory C-type lectin receptor signaling pathway protein phosphorylation exocytosis apoptotic process axonogenesis positive regulation of cell proliferation positive regulation of cell migration T cell costimulation actin cytoskeleton reorganization cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation positive regulation of intracellular estrogen receptor signaling pathway Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis wound healing positive regulation of JUN kinase activity protein autophosphorylation ephrin receptor signaling pathway branching morphogenesis of an epithelial tube neuron projection morphogenesis T cell receptor signaling pathway positive regulation of stress fiber assembly negative regulation of cell proliferation involved in contact inhibition negative regulation of protein kinase activity signal transduction viral process phosphorylation peptidyl-serine phosphorylation signal transduction by protein phosphorylation regulation of growth negative regulation of apoptotic process vascular endothelial growth factor receptor signaling pathway regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation dendritic spine development positive regulation of protein tyrosine kinase activity cellular response to organic cyclic compound cell-cell adhesion positive regulation of extrinsic apoptotic signaling pathway negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis Rho protein signal transduction regulation of mitotic cell cycle neuromuscular junction development metabolic process response to organic substance regulation of gene expression dendrite development cell migration amygdala development actin cytoskeleton organization stress-activated protein kinase signaling cascade activation of protein kinase activity regulation of actin cytoskeleton organization regulation of apoptotic process receptor clustering regulation of MAPK cascade gamma-aminobutyric acid secretion, neurotransmission glutamate secretion, neurotransmission observational learning regulation of long-term synaptic potentiation activation of MAPKK activity small GTPase mediated signal transduction intracellular signal transduction
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