Western blot analysis of Phospho-PAK1 pThr84 in mouse hippocampal lysate using a Phospho-PAK1 pThr84 polyclonal antibody (Product # PA5-35408). Results show a band at ~68kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in Lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 of human PAK1 conjugated to KLH|
|Storage buffer||0.1M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 0.1mg/ml BSA|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, canine, chicken, non-human primate, rat, sheep and zebrafish based on 100% sequence homology.
This antibody contains enough material to conduct 10 mini-Western blots.
This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.