Western blot analysis of Phospho-PAK3 pSer154 using Phospho-PAK3 pSer154 polyclonal antibody (Product # PA5-36866) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1ng/ml, 30min), Lane 2: sp2/0 cell lysate treated with EGF (0.1ng/ml, 30min), Lane 3: H9C2 cell lysate treated with EGF (0.1ng/ml, 30min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human PAK3 around the phosphorylation site of Serine 154|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||15mM sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 65 kDa.
Purity is >95% by SDS-PAGE.
Three isoforms of serine/threonine kinases, designated alphaPAK p68, betaPAK p65 and gammaPAK p62, have been shown to exhibit a high degree of sequence homology with the S. cerevisiae kinase Ste 20, involved in pheromone signaling. The alpha, beta and gammaPAK isoforms complex specifically with Rac1 and Cdc42 in their active GTP-bound state, inhibiting their intrinsic GTPase activity leading to their autophosphorylation. There are eight sites of autophosphorylation on gammaPAK, including Ser 19, Ser 141 and Thr 402, and phosphorylation of Ser 141 and Thr 402 is correlated with gammaPAK activation. Once phosphorylated and their affinity for Rac/Cdc42 reduced, the PAK isoforms disassociate from the complex to seek downstream substrates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.