Western blot analysis of Phospho-PAK4+PAK5+PAK6 pSer474 using Phospho-PAK4+PAK5+PAK6 pSer474 polyclonal antibody (Product # PA5-36865) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with EGF (0.1ng/ml, 30min), Lane 2: Raw264.7 cell lysate treated with EGF (E0.1ng/ml, 30min), Lane 3: PC12 cell lysate treated with EGF (0.1ng/ml, 30min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human PAK4 around the phosphorylation site of Serine 474|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 72 kDa (PAK4), 82 kDa (PAK6) and 90 (PAK7).
Purity is >95% by SDS-PAGE.
PAK4 is highly expressed in prostate, testis and colon. PAK4 interacts tightly with GTP-bound but not GDP-bound CDC42 and weakly with RAC. PAK4 phosphorylates and autophosphorylates and also activates the JNK pathway. PAK5, a member of the PAK family of protein kinases contains a CDC42/Rac1 interactive binding (CRIB) motif at the N-terminus and a Ste20-like kinase domain at the C-terminus. PAK5 preferentially binds to CDC42 in the presence of GTP and the CRIB motif is essential for this interaction.PAK6 protein cotranslocates into the nucleus with androgen receptor, which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development, in response to androgen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.