Western blot analysis of Phospho-PAR-4 pThr163 in extracts from HeLa cells, untreated or treated with a phosphatase inhibitor (100 nM, 5 minutes), using Phospho-PAR-4 pThr163 polyclonal antibody (Product # PA5-17343) (upper) or a PAR-4 polyclonal antibody (lower).
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding pThr163 of human PAR-4 (Thr155 in rat and pThr156 in mouse)|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
The tumor suppressor WT1 represses and activates transcription. The protein encoded by this gene is a WT1-interacting protein that itself functions as a transcriptional repressor. It contains a putative leucine zipper domain which interacts with the zinc finger DNA binding domain of WT1. This protein is specifically upregulated during apoptosis of prostate cells.
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