Invitrogen

Phospho-PAX2 (Ser393) Polyclonal Antibody

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Phospho-PAX2 (Ser393) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Pax2 (pSer393) showing nucleus and weak cytoplasmic staining of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-Pax2 (pSer393) Polyclonal Antibody (Product # 36-9200) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-PAX2 (Ser393) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MDA-MB-231 (Lane 2), LNCaP (lane 3) and PC-12 (Lane 4). The blots were probed with Anti-Pax (pS393) Rabbit Polyclonal Antibody (Product # 36-9200, 1-3 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 42 kDa band corresponding to Pax (pS393) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

36-9200

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

ELISA (ELISA)

Assay-dependent

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Ser393 of the 415 amino acid mouse Pax2 isoform is equivalent to Ser394 of the 416 amino acid human Pax2 isoform, and to Ser371 of the 393 amino acid human Pax2 isoform.

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533286

Target Information

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Regulating the dorsal neural tube expression of Ptf1a through a distal 3' enhancer.

Developmental biology

Mona B,Avila JM,Meredith DM,Kollipara RK,Johnson JE

36-9200 was used in immunohistochemistry - frozen section to assess dorsalneural tube expression of Ptf1a regulated through a distal 3' enhancer

Sat Oct 01 00:00:00 EDT 2016

BMP7 and SHH regulate Pax2 in mouse retinal astrocytes by relieving TLX repression.

Developmental biology

Sehgal R,Sheibani N,Rhodes SJ,Belecky Adams TL

Sat Aug 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: optic disc coloboma; paired box gene 2; paired box homeotic gene 2; Paired box protein Pax-2

Gene Aliases: FSGS7; Opdc; PAPRS; Pax-2; PAX2

UniProt ID: (Human) Q02962, (Mouse) P32114

Entrez Gene ID: (Human) 5076, (Mouse) 18504, (Rat) 293992

Function(s)

Process(es)

core promoter proximal region sequence-specific DNA binding DNA binding protein binding transcription factor binding superoxide-generating NADPH oxidase activity transcription regulatory region DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding C2H2 zinc finger domain binding sequence-specific DNA binding