Invitrogen

Phospho-PAX2 (Ser393) Polyclonal Antibody

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Phospho-PAX2 (Ser393) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Pax2 (pSer393) showing nucleus and weak cytoplasmic staining of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-Pax2 (pSer393) Polyclonal Antibody (Product # 36-9200) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-PAX2 (Ser393) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Pax2 (pSer393) showing staining in the nucleus of paraffin-embedded human fallopian tube tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-Pax2 (pSer393) Polyclonal Antibody (Product # 36-9200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-PAX2 (Ser393) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Phospho-Pax2 (pSer393) showing staining in the nucleus of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Phospho-Pax2 (pSer393) Polyclonal Antibody (Product # 36-9200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Phospho-PAX2 (Ser393) Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), MDA-MB-231 (Lane 2), LNCaP (lane 3) and PC-12 (Lane 4). The blots were probed with Anti-Pax (pS393) Rabbit Polyclonal Antibody (Product # 36-9200, 1-3 µg/mL) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 42 kDa band corresponding to Pax (pS393) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Phospho-PAX2 (Ser393) Antibody in Western Blot (WB) — Western blot analysis of (A) phospho-Pax2-GST fusion protein, (B) Pax2-GST fusion protein, and (C) HEK293 cell lysates using Rb anti-phospho-Pax2 (Ser393) (Product # 36-9200).

Product Details

Applications

Tested Dilution

Publications

ELISA (ELISA)

Assay Dependent

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Western Blot (WB)

Assay Dependent

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phospho-peptide derived from the sequence surrounding the phosphorylated Ser393 residue of mouse and human Pax2 (paired box protein 2). Ser393 of the 415 amino acid mouse Pax2 isoform is equivalent to Ser394 of the 416 amino acid human Pax2 isoform, and to Ser371 of the 393 amino acid human Pax2 isoform.

Conjugate

Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

0.1% sodium azide

Storage conditions

-20°C

RRID

AB_2533286

Target Information

The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: optic disc coloboma; paired box gene 2; paired box homeotic gene 2; Paired box protein Pax-2

Gene Aliases: FSGS7; Opdc; PAPRS; Pax-2; PAX2

UniProt ID: (Human) Q02962, (Mouse) P32114

Entrez Gene ID: (Human) 5076, (Mouse) 18504, (Rat) 293992

Molecular Function: DNA binding protein helix-turn-helix transcription factor homeobox transcription factor nucleic acid binding transcription factor

urogenital system development branching involved in ureteric bud morphogenesis cell fate determination mesonephros development neural tube closure optic cup morphogenesis involved in camera-type eye development mesenchymal to epithelial transition involved in metanephros morphogenesis retinal pigment epithelium development transcription, DNA-templated transcription from RNA polymerase II promoter axonogenesis mesodermal cell fate specification aging visual perception glial cell differentiation optic nerve development optic nerve morphogenesis optic nerve structural organization vestibulocochlear nerve formation response to nutrient levels regulation of metanephros size ureter maturation pronephric field specification inner ear morphogenesis camera-type eye development negative regulation of apoptotic process negative regulation of programmed cell death negative regulation of cysteine-type endopeptidase activity involved in apoptotic process protein kinase B signaling negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of cytolysis positive regulation of transcription from RNA polymerase II promoter pronephros development brain morphogenesis stem cell differentiation positive regulation of epithelial cell proliferation oxidation-reduction process mesenchymal to epithelial transition optic chiasma development cellular response to hydrogen peroxide cellular response to retinoic acid cellular response to glucose stimulus cellular response to epidermal growth factor stimulus metanephric mesenchyme development positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis metanephric mesenchymal cell differentiation nephric duct formation ureter development metanephric collecting duct development metanephric epithelium development metanephric distal convoluted tubule development metanephric nephron tubule formation positive regulation of metanephric glomerulus development negative regulation of mesenchymal cell apoptotic process involved in metanephric nephron morphogenesis regulation of metanephric nephron tubule epithelial cell differentiation reactive oxygen species metabolic process cochlea development cochlea morphogenesis positive regulation of branching involved in ureteric bud morphogenesis negative regulation of mesenchymal cell apoptotic process involved in metanephros development negative regulation of apoptotic process involved in metanephric collecting duct development negative regulation of apoptotic process involved in metanephric nephron tubule development negative regulation of reactive oxygen species metabolic process positive regulation of metanephric DCT cell differentiation positive regulation of optic nerve formation negative regulation of transcription from RNA polymerase II promoter metanephros development ureteric bud development kidney development regulation of transcription, DNA-templated multicellular organism development positive regulation of cell proliferation cell differentiation regulation of apoptotic process regulation of programmed cell death animal organ development paramesonephric duct development renal system development mesonephric tubule development mesonephric tubule formation mesonephric duct development pancreas development cellular response to mechanical stimulus ureter morphogenesis

Process(es)

core promoter proximal region sequence-specific DNA binding DNA binding protein binding transcription factor binding superoxide-generating NADPH oxidase activity transcription regulatory region DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding C2H2 zinc finger domain binding sequence-specific DNA binding