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Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of mouse PAX2. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.57 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2792895 |
Keep as concentrated solution.
Predicted reactivity: Mouse (100%), Rat (100%), Sheep (100%).
Positive Control: HCT116, HCT116 nuclear extract.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
The pax genes are a family of transcription factors that are active in specific tissues during early embryonic development. Pax family members possess a DNA-binding domain encoded by the paired box. Because paired domain containing genes encode transcription factors, they are capable of executing a genetic program. Several pax genes have also been associated with developmental mutations including: pax-3, which is associated with Waardenburg syndrome, pax-6, which is associated with Aniridia, and pax-2, which is associated with Wilms tumor. During embryogenesis, Pax-2 is expressed in the developing kidney. In particular, the pax-2 gene is expressed in condensing metanephric mesenchyme and in early epithelial structures derived from mesenchyme; however, pax-2 mRNA and protein levels are rapidly down regulated as the tubular epithelium matures. Although Pax-2 is down regulated during renal epithelium maturation, Pax-2 expression persists in the undifferentiated epithelium of Wilms' tumors. Persistent expression of Pax-2 in Wilms' tumors occurs frequently and correlates with the proliferation of poorly differentiated epithelial cells in these tumors. Interestingly, expression of the Wilms' tumor suppresser protein, WT1, coincides with down-regulation of the pax-2 gene; thus, suggesting that WT1 can directly repress pax-2 transcription.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: optic disc coloboma; paired box gene 2; paired box homeotic gene 2; Paired box protein Pax-2
Gene Aliases: FSGS7; Opdc; PAPRS; Pax-2; PAX2
UniProt ID: (Human) Q02962, (Mouse) P32114
Entrez Gene ID: (Human) 5076, (Mouse) 18504
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