Western blot analysis of Phospho-PDGF Receptor alpha pTyr1018 in cell extracts from NIH/3T3 cells, untreated or treated with PDGF-AA, using Phospho-PDGF Receptor alpha pTyr1018 polyclonal antibody (Product # PA5-17070) (upper) or a PDGF Receptor alpha polyclonal antibody (lower).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to residues surrounding pTyr1018 of human PDGFRa|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with activated PDGFR-beta.
This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.