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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PDGFRA/PDGFRB Antibodies

          Invitrogen

          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Polyclonal Antibody

          Advanced Verification
          1 Published Figure
          3 References
          View all (2) PDGFRA/PDGFRB antibodies

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          Cite Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Polyclonal Antibody

          • Antibody Testing Data (2)
          • Published Figures (1)
          • Advanced Verification (1)
          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody (44-1000G) in ICC/IF

          Immunofluorescence analysis of PDGFR alpha + beta (pYpY572/574) was done on 70% confluent log phase MCF7 cells treated with PDGF (50 ng/mL for 10 min). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with PDGFR alpha + beta (pYpY572/574) Rabbit polyclonal Antibo... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody in Immunocytochemistry (ICC/IF)
          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody in Western Blot (WB)
          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody in Western Blot (WB)
          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Antibody
          Phospho-PDGFRA/PDGFRB (Tyr572, Tyr574) Polyclonal Antibody

          Product Details

          44-1000G

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 1 publication 1 publication

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human PDGFRA that contains tyrosines 572 and 574 and PDGFRB tyrosines 579 and 581. The sequence is conserved in PDGFRA mouse, rat and frog.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Antigen affinity chromatography

          Storage buffer

          Dulbecco's PBS, pH 7.3, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2533550

          Target Information

          PDGFRA is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. Studies in knockout mice, where homozygosity is lethal, indicate that the alpha form of the platelet-derived growth factor receptor is particularly important for kidney development since mice heterozygous for the receptor exhibit defective kidney phenotypes.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Alpha platelet-derived growth factor receptor; Alpha platelet-derived growth factor receptor precursor (PDGF-R-alpha); Alpha-type platelet-derived growth factor receptor; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member A; CD140 antigen-like family member B; CD140a; CD140a antigen; CD140b; MGC74795; PDGF alpha chain; PDGF beta chain; PDGF Receptor alpha; PDGF-R-alpha; PDGF-R-beta; PDGFR-1; PDGFR-2; pdgfr-a; PDGFR-alpha; PDGFR-beta; PDGFRA/BCR fusion; PGFRB; Platelet-derived growth factor alpha receptor; Platelet-derived growth factor receptor 1; Platelet-derived growth factor receptor 2; Platelet-derived growth factor receptor alpha; Platelet-derived growth factor receptor beta; platelet-derived growth factor receptor beta variant 1; platelet-derived growth factor receptor, alpha polypeptide; platelet-derived growth factor receptor, beta polypeptide; rearranged-in-hypereosinophilia-platelet derived growth factor receptor alpha fusion protein

          View more View less

          Gene Aliases: AI115593; AI528809; CD140A; CD140B; GAS9; IBGC4; IMF1; JTK12; KOGS; PDGFR; PDGFR-1; PDGFR-2; PDGFR1; PDGFR2; PDGFRA; PDGFRB; PENTT; RHEPDGFRA

          View more View less

          UniProt ID: (Human) P16234, (Human) P09619, (Mouse) P26618, (Mouse) P05622

          View more View less

          Entrez Gene ID: (Human) 5156, (Human) 5159, (Mouse) 18595, (Mouse) 18596

          View more View less

          Function(s)
          protein kinase activity transmembrane receptor protein tyrosine kinase activity platelet-derived growth factor alpha-receptor activity vascular endothelial growth factor-activated receptor activity Ras guanyl-nucleotide exchange factor activity platelet-derived growth factor receptor binding protein binding ATP binding vascular endothelial growth factor binding protein homodimerization activity phosphatidylinositol-4,5-bisphosphate 3-kinase activity platelet-derived growth factor binding protein tyrosine kinase activity platelet activating factor receptor activity platelet-derived growth factor-activated receptor activity platelet-derived growth factor beta-receptor activity receptor binding protein kinase binding phosphatidylinositol 3-kinase binding nucleotide binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein complex binding signal transducer activity transmembrane signal receptor
          Process(es)
          MAPK cascade luteinization cell activation positive regulation of cytosolic calcium ion concentration positive regulation of cell proliferation negative regulation of platelet activation positive regulation of phospholipase C activity regulation of phosphatidylinositol 3-kinase signaling positive regulation of phosphatidylinositol 3-kinase signaling viral process peptidyl-tyrosine phosphorylation positive regulation of cell migration cellular response to reactive oxygen species platelet-derived growth factor receptor-alpha signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway wound healing positive regulation of GTPase activity positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication protein autophosphorylation phosphatidylinositol phosphorylation platelet-derived growth factor receptor signaling pathway phosphatidylinositol-mediated signaling positive regulation of fibroblast proliferation embryonic digestive tract morphogenesis embryonic cranial skeleton morphogenesis embryonic skeletal system morphogenesis regulation of chemotaxis cardiac myofibril assembly cell chemotaxis retina vasculature development in camera-type eye positive regulation of ERK1 and ERK2 cascade platelet aggregation metanephric glomerular capillary formation regulation of actin cytoskeleton reorganization regulation of mesenchymal stem cell differentiation glycosaminoglycan biosynthetic process signal transduction G-protein coupled receptor signaling pathway response to toxic substance positive regulation of smooth muscle cell migration cell migration response to estradiol positive regulation of phosphoprotein phosphatase activity response to retinoic acid regulation of actin cytoskeleton organization positive regulation of collagen biosynthetic process response to fluid shear stress cell migration involved in vasculogenesis metanephric mesenchymal cell migration platelet-derived growth factor receptor-beta signaling pathway positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway aorta morphogenesis cellular response to platelet-derived growth factor stimulus response to hydrogen peroxide negative regulation of apoptotic process positive regulation of MAP kinase activity response to estrogen positive regulation of mitotic nuclear division phosphatidylinositol metabolic process positive regulation of smooth muscle cell proliferation inner ear development positive regulation of chemotaxis response to hyperoxia cell migration involved in coronary angiogenesis smooth muscle cell chemotaxis metanephric mesenchyme development metanephric glomerular mesangial cell proliferation involved in metanephros development metanephric comma-shaped body morphogenesis metanephric S-shaped body morphogenesis positive regulation of calcium ion import positive regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process in utero embryonic development hematopoietic progenitor cell differentiation protein phosphorylation chemotaxis transmembrane receptor protein tyrosine kinase signaling pathway multicellular organism development estrogen metabolic process female gonad development anatomical structure morphogenesis organ morphogenesis phosphorylation signal transduction involved in regulation of gene expression extracellular matrix organization lung development adrenal gland development male genitalia development Leydig cell differentiation odontogenesis of dentin-containing tooth skeletal system morphogenesis palate development face morphogenesis cellular response to amino acid stimulus kidney development tissue homeostasis nitrogen compound metabolic process hemopoiesis intracellular signal transduction smooth muscle tissue development regulation of peptidyl-tyrosine phosphorylation cardiac vascular smooth muscle cell differentiation
          It has to be done as per old AB suggested Products section.
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