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Western blot analysis of Phospho-PFKFB2 pSer483 using Phospho-PFKFB2 pSer483 polyclonal antibody (Product # PA5-36869) at a dilution of 1:500. Lane 1: Hela cell lysate treated with H2O2 (100uM, 30min), Lane 2: sp2/0 cell lysate treated with H2O2 (100uM, 30min), Lane 3: H9C2 cell lysate treated with H2O2 (100uM, 30min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human PFKFB2 around the phosphorylation site of Serine 483|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 58 kDa.
Purity is >95% by SDS-PAGE.
PFK-1 undergoes activation in the presence of elevated AMP. The most potent activator is fructose-2,6-bisphosphate, which is produced by PFK-2 from the same substrate, fructose 6-phosphate. PFK-2 is bifunctional and a key regulator for PFK-1. PFK-2 catalyzes the synthesis of fructose-2,6-bisphosphate, and contains fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. PFK-2 is dimeric and isoenzymes include PFK-2 liver (PFKFB1, PFRX), PFK-2 cardiac (PFKFB2), PFK-2 placental (PFKFB3, inducible PFK-2) and PFK-2 testis (PFKFB4).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
6-bisphosphatase; 6-bisphosphatase 2; 6-P2ase 2; 6-P2ase heart-type isozyme; 6-phosphofructo-2-kinase; 6-phosphofructo-2-kinase/fructose-2; 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2; 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (heart); 6PF-2-K/Fru-2; 6PF-2-K/Fru-2,6-P2ase 2; 6PF-2-K/Fru-2,6-P2ASE heart-type isozyme; fructose 6-phosphate 2-kinase/fructose 2,6-bisphosphatase; Fructose-2; fructose-2,6-bisphosphatase, cardiac isozyme; PFK-2 car; PFK-2 cardiac; PFK-2/FBPase-2; PFK-2/FBPase-2 gene B; PFK/FBPase 2; PFKFB, cardiac; PFKFB2
4930568D07Rik; PFK-2/FBPase-2; PFKFB2; RH2K