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Phospho-PI 3 Kinase p85 alpha (Tyr607) Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (2)

Phospho-PI 3 Kinase p85 alpha (Tyr607) Antibody in Immunohistochemistry (IHC)

FIGURE: 1 / 2

Phospho-PI 3 Kinase p85 alpha (Tyr607) Antibody (600-401-MR1) in IHC

Immunohistochemistry of Rabbit Anti-PI 3 Kinase p85 alpha pY607 Antibody. Tissue: Human Brain Cortex. Fixative: none. Antigen Retrieval: HIER using Citrate Buffer for 20 minutes. Primary Antibody: Anti-PI3 Kinase p85 alpha pY607 at 1:100 for 30mins at RT. Secondary Antibody: Goat Anti-Rabbit Poly IgG HRP Ready-to-Use for 8 mins at RT. Counterstain: Hematoxylin. Substrate: DAB. Results: shows intense... View More

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Phospho-PI 3 Kinase p85 alpha (Tyr607) Antibody in Dot Blot (DB) — Dot Blot of Rabbit Anti-PI 3 Kinase p85 alpha pY607 Antibody. Dilution Columns: 1-100ng, 2-33.33ng, 3-11.11ng, 4-3.70ng, 5-1.23ng. Row A: PI 3 Kinase p85 alpha pY607-BSA conjugated Peptide. Row B: PI 3 Kinase p85 alpha non-phospho Y607-BSA conjugated Peptide. Row C: STAT3 pY705-BSA conjugated Peptide. Row D: STAT3 non-phospho Y705-BSA conjugated Peptide. Row E: Scrambled Sprouty-BSA conjugated peptide. Row F: BSA only. Primary Antibody: Anti-PI 3 Kinase p85 alpha pY607 at 1µg/mL overnight at 2-8°C. Secondary Antibody: Goat Anti-Rabbit IgG HRP (p/n 611-103-122) 1:70,000 for 1hr at RT. Blocking: Universal BlockOut Buffer (p/n MB-073). Exposure: 10 sec.

Product Details

600-401-MR1

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

Immunohistochemistry (IHC)

1:100

ELISA (ELISA)

1:10,000-1:50,000

Dot blot (DB)

1:70,000

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Anti-PI 3 Kinase p85 alpha pY607 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Terminal portion of human PIK3R1 conjugated to Keyhole Limpet Hemocyanin (KLH).

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.8 mg/mL

Purification

Affinity chromatography

Storage buffer

0.02M potassium phosphate, pH 7.2, with 0.15M NaCl

Contains

0.01% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

This affinity purified antibody is directed against the phosphorylated form of human PIK3R1 protein at the pY607 residue. Reactivity with non-phosphorylated human PIK3R1 Y607 is minimal by ELISA and dot blot. Reactivity against homologues from other sources is not known. A BLAST analysis was used to suggest reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, and rat.

Target Information

Phosphatidylinositol 3-kinase (PIK3) is a signaling protein that phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. Signaling is initiated when upstream tyrosine kinase receptors or G protein coupled receptors are bound by their ligands. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1. Mutations in the gene can result in Agammaglobulinemia 7, SHORT syndrome, or Immunodeficiency 36.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: p55-GAMMA; phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); PI3-kinase p85-subunit alpha; PI3-kinase regulatory subunit alpha; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 1 p85 alpha; ptdIns-3-kinase regulatory subunit alpha; PtdIns-3-kinase regulatory subunit p85-al; RP4-533D7.2

Gene Aliases: AGM7; GRB1; IMD36; p85; p85-ALPHA; PIK3R1

UniProt ID: (Human) P27986

Entrez Gene ID: (Human) 5295

Molecular Function: kinase modulator

Function(s)

Process(es)

transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity