|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide sequence around phosphorylation site of tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85/p55 .|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
A suggested positive control for Western blot is 293 cells.
Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110kDa catalytic subunit and a regulatory subunit of either 85, 55, or 50kDa. It binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. During insulin stimulation, it also binds to IRS-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 100% homology to SWISS-PROT Q92569; ga; gamma); p55-GAMMA; p55PIK; phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55); phosphatidylinositol 3-kinase; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; phosphatidylinositol 3-kinase p55 subunit; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase-associated p-85 alpha; Phosphoinositide 3-kinase p85 (other splicing variants: p55 and p50); Phosphoinositide 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphoinositide-3-kinase; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3-kinase p85 subunit alpha; PI3-kinase p85 subunit gamma; PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3K regulatory subunit alpha; PI3K regulatory subunit gamma; polypeptide 3 (p55; ptdIns-3-kinase p85-alpha; ptdIns-3-kinase p85-gamma; PtdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-alpha; regulatory subunit; regulatory subunit 3 (p55; RP4-533D7.2
Gene Aliases: AA414954; AGM7; GRB1; IMD36; p50alpha; p55; p55-GAMMA; p55alpha; p55PIK; p85; p85-ALPHA; p85alpha; PI3K; PI3KA; PIK3R1; PIK3R3
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.