|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from a region of human PKC beta 2 that contains threonine 641.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-22053 detects Phospho-PKC beta 2 (pT641) in Human samples.
PA1-22053 has been successfully used in Western Blot procedures.
PA1-22053 immunogen corresponds to Synthetic phosphopeptide derived from a region of human PKC beta 2 that contains threonine 641..
Protein Kinase C beta 2 (PKC beta 2) is an 80 kDa member of the conventional group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion. PKC beta 2 is phosphorylated on three sites, threonine 500 in the activation loop, threonine 641 in the turn loop and serine 660 in the hydrophobic loop. Threonine 641 mediates PKC beta 2 binding to Hsp70 which regulates its stability and phosphorylation. Phosphorylation of threonine 641 is also crucial for PKC beta 2 proper subcellular localization and catalytic function as well as for its autophosphorylation on serine 660.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.