Invitrogen

Phospho-PKC delta (Ser645) Polyclonal Antibody

View all (48) PKC delta antibodies
Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

PA5-36777

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of Serine 645
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2553724

Product Specific Information

This antibody detects endogenous protein at a molecular weight of 80 kDa.

Purity is >95% by SDS-PAGE.

Target Information

The PKC family of serine/threonine kinases, including PRKCD (PKC delta), is activated intracellularly by signal transduction pathways. In humans, at least 12 different PKC polypeptides have been identified. These isoforms differ in primary structure, tissue distribution, subcellular localization, mode of action in vitro, response to extracellular signals, and substrate specificity. PKC alpha, beta I, beta II, and gamma form the conventional family; their activities are Ca2+- and phospholipid-dependent. Protein kinase C (PKC) can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 1-May; kinase nPKC-delta; KPCD; nPKC-delta; Protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta; Tyrosine-protein kinase PRKCD

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Gene Aliases: ALPS3; CVID9; MAY1; nPKC-delta; PKCD; PRKCD

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UniProt ID: (Human) Q05655

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Entrez Gene ID: (Human) 5580

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Function(s)
protein kinase activity protein serine/threonine kinase activity protein kinase C activity calcium-independent protein kinase C activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding enzyme activator activity enzyme binding kinase binding protein kinase binding insulin receptor substrate binding metal ion binding non-receptor serine/threonine protein kinase
Process(es)
stimulatory C-type lectin receptor signaling pathway protein phosphorylation apoptotic process cellular component disassembly involved in execution phase of apoptosis cell cycle signal transduction activation of phospholipase C activity intrinsic apoptotic signaling pathway in response to oxidative stress regulation of receptor activity immunoglobulin mediated immune response histone phosphorylation peptidyl-serine phosphorylation peptidyl-threonine phosphorylation peptidyl-tyrosine phosphorylation termination of signal transduction platelet activation negative regulation of actin filament polymerization positive regulation of endodeoxyribonuclease activity negative regulation of protein binding activation of protein kinase activity interleukin-10 production interleukin-12 production positive regulation of superoxide anion generation regulation of actin cytoskeleton organization negative regulation of glial cell apoptotic process positive regulation of protein dephosphorylation Fc-gamma receptor signaling pathway involved in phagocytosis B cell proliferation neutrophil activation positive regulation of protein import into nucleus defense response to bacterium negative regulation of MAP kinase activity regulation of mRNA stability negative regulation of insulin receptor signaling pathway negative regulation of inflammatory response negative regulation of peptidyl-tyrosine phosphorylation protein stabilization negative regulation of filopodium assembly cell chemotaxis interferon-gamma-mediated signaling pathway cellular response to hydrogen peroxide cellular response to hydroperoxide negative regulation of platelet aggregation cellular senescence positive regulation of phospholipid scramblase activity cellular response to angiotensin positive regulation of ceramide biosynthetic process positive regulation of glucosylceramide catabolic process positive regulation of sphingomyelin catabolic process positive regulation of response to DNA damage stimulus positive regulation of apoptotic signaling pathway
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