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|Tested species reactivity||Human, Mouse|
|Published species reactivity||Virus, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human PKCe that contains serine 729. The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of melanoma growth by small molecules that promote the mitochondrial localization of ATF2.
44-977G was used in western blot to discuss the consequences of cytoplasmic ATF2 in melanoma cells.
|Varsano T,Lau E,Feng Y,Garrido M,Milan L,Heynen-Genel S,Hassig CA,Ronai ZA||Clinical cancer research : an official journal of the American Association for Cancer Research (19:2710)||2013|
|Virus||Not Cited||Roles of protein kinase C isotypes during seawater-versus cAMP-induced oocyte maturation in a marine worm.||Stricker SA||Molecular reproduction and development (76:693)||2009|
nPKC-epsilon; PKC e; PKCE; protein kinase C epsilon type; protein kinase C, epsilon
5830406C15Rik; nPKC-epsilon; PKC[e]; PKCE; Pkcea; PKCepsilon; PRKCE; R75156