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Invitrogen
Phospho-PKC theta (Thr538) Recombinant Rabbit Monoclonal Antibody (F4H4L1)
Advanced Verification
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
Phospho-PKC theta (Thr538) Antibody (700043) in IHC (P)
Immunohistochemistry analysis of Phospho-PKC theta pThr538 in formalin-fixed, paraffin-embedded human breast carcimona tissue using a Phospho-PKC theta pThr538 monoclonal antibody (Product # 700043) at a dilution of 5 µg/mL. Staining was visualized using DAB. Images were taken at a magnification of 20x. Results show cytoplasmic staining in tumor cells.
Product Details
700043
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
F4H4L1
Immunogen
A peptide corresponding to amino acids 531-539 of Q04759.
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID
AB_2532274
Product Specific Information
This antibody is predicted to react with bovine, chimpanzee, mouse, rat and Xenopus based on sequence homology.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
Protein Kinase C (PKC) isoforms are serine/threonine kinases involved in signal transduction pathways that govern a wide range of physiological processes including differentiation, proliferation, gene expression, brain function, membrane transport and the organization of cytoskeletal and extracellular matrix proteins. Increasing evidence from studies using in vitro and in vivo systems points to PKC as a key regulator of critical cell cycle transitions, including cell cycle entry and exit and the G1 and G2 checkpoints. PKC-mediated control of these transitions can be negative or positive, depending on the timing of PKC activation during the cell cycle and on the specific PKC isozymes involve. There have been at least 12 different PKC isoforms identified in humans to date, including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. The different PKC isoforms vary in structure, subcellular localization, tissue distribution and response to extracellular signals. PKC beta II has been shown to be expressed, in part, in cardiac muscle, intestinal epithelium, kidney and pancreas. PKC beta II has been shown to be involved in cell cycle progression and cancer proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: EC 2.7.11.13; kinase nPKC-theta; KPCT; MGC126514; MGC141919; nPKC-theta; Protein kinase C theta type; protein kinase C, theta; RP11-563J2.1
Gene Aliases: nPKC-theta; PRKCQ; PRKCT
UniProt ID: (Human) Q04759
Entrez Gene ID: (Human) 5588
Molecular Function:
non-receptor serine/threonine protein kinase
regulation of cell growth
response to hypoxia
regulation of transcription, DNA-templated
membrane protein ectodomain proteolysis
cellular component disassembly involved in execution phase of apoptosis
inflammatory response
axon guidance
aging
response to heat
response to glucose
regulation of G2/M transition of mitotic cell cycle
response to organic cyclic compound
peptidyl-serine phosphorylation
regulation of vasoconstriction
platelet activation
positive regulation of telomere maintenance via telomerase
positive regulation of interleukin-17 production
positive regulation of interleukin-4 production
response to insulin
intracellular signal transduction
Fc-epsilon receptor signaling pathway
positive regulation of T cell proliferation
tissue regeneration
positive regulation of NF-kappaB import into nucleus
positive regulation of interleukin-2 biosynthetic process
respiratory burst
negative regulation of insulin receptor signaling pathway
positive regulation of protein secretion
T cell receptor signaling pathway
positive regulation of T cell activation
positive regulation of NF-kappaB transcription factor activity
positive regulation of filopodium assembly
positive regulation of stress fiber assembly
positive regulation of telomerase activity
cell chemotaxis
negative regulation of T cell apoptotic process
regulation of platelet aggregation
positive regulation of telomere capping
positive regulation of T-helper 17 type immune response
positive regulation of T-helper 2 cell activation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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