Western blot analysis of Phospho-PKC theta pSer695 using 30µg of A) HeLa (B) HepG2 and C) MOLT4 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a Phospho-PKC theta pSer695 polyclonal antibody (Product # PA5-29029) at a dilution of 1:1000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 641 and 702 of Human PKC theta-phospho-S695|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29029 targets Phospho-PKC theta pSer695 in WB applications and shows reactivity with Human samples.
The PA5-29029 immunogen is synthetic peptide corresponding to a region within amino acids 641 and 702 of Human PKC theta-phospho-S695.
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors.
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