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Immunohistochemistry analysis of Phospho-PKC theta pThr538 in formalin-fixed, paraffin-embedded human breast carcimona tissue using a Phospho-PKC theta pThr538 monoclonal antibody (Product # 700043) at a dilution of 5ug/ml. Staining was visualized using DAB. Images were taken at a magnification of 20x. Results show cytoplasmic staining in tumor cells.
|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 531-539 of Q04759.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||4-6 ug/ml|
|Western Blot (WB)||2-4 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
This antibody is predicted to react with bovine, chimpanzee, mouse, rat and Xenopus based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Protein Kinase C theta of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and homeostasis. Transgenic mice over-expressing dominant negative PKC theta develop hyperinsulinimia. PKC theta is involved in JNK activation and also plays a specialized role in TCR-mediated activation of T and B cells. Through its control of Rap1, PKC theta establishes a threshold for T cell activation and positively regulates cytokine response and adhesive properties. The activation of PKC theta in T cells is associated with its recruitment to the membrane, and is mediated by PI3-kinase and Vav. PKC theta is a constitutively competent kinase and is phosphorylated on threonine 538 in the activation loop. The phosphorylation of threonine 538 is critical to PKC theta kinase activity, and plays an important role in PKC theta-mediated activation of nuclear factor kappaB.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Inhibition of PKC-θ preserves cardiac function and reduces fibrosis in streptozotocin-induced diabetic cardiomyopathy.
700043 was used in immunohistochemistry - paraffin section to test if inhibition of PKC-θ protect diabetic hearts by suppressing T-cell stimulation and maintaining tight junction integrity.
|Li Z,Abdullah CS,Jin ZQ||British journal of pharmacology (171:2913)||2014|
nPKC-theta, RP11-563J2.1, PRKCT
protein kinase C theta type, RP11-563J2.1, MGC126514, MGC141919, PRKCT, PRKCQ