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Phospho-PKN1 (Thr774) Antibody (29107-1-AP100UL) in WB
Non-treated and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with (Product # 29107-1-AP100UL) (Phospho-PKN1 (Thr774) antibody) at dilution of 1:5,000 incubated at room temperature for 1 hours.
Product Details
29107-1-AP100UL
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Conjugate
Form
Liquid
Concentration
0.3 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
Target Information
PKN1 (PRK1) belongs to the protein kinase C superfamily. This kinase is activated by the Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can also be activated by phospholipids. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PAK-1; Protease-activated kinase 1; Protein kinase C-like 1; Protein kinase C-like PKN; protein kinase C-related kinase 1; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine kinase 3; serine/threonine protein kinase N; Serine/threonine-protein kinase N1
Gene Aliases: DBK; F730027O18Rik; PAK-1; PAK1; PKN; PKN-ALPHA; PKN1; PRK1; PRKCL1; Stk3
UniProt ID: (Human) Q16512, (Mouse) P70268
Entrez Gene ID: (Human) 5585, (Mouse) 320795
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein kinase C binding
protein binding
ATP binding
GTP-Rho binding
ligand-dependent nuclear receptor transcription coactivator activity
histone kinase activity (H3-T11 specific)
histone binding
histone deacetylase binding
Rac GTPase binding
androgen receptor binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
B cell homeostasis
B cell apoptotic process
regulation of germinal center formation
regulation of immunoglobulin production
renal system process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
negative regulation of protein kinase activity
hyperosmotic response
signal transduction
activation of JUN kinase activity
epithelial cell migration
peptidyl-serine phosphorylation
negative regulation of B cell proliferation
histone H3-T11 phosphorylation
spleen development
regulation of cell motility
negative regulation of protein phosphorylation
regulation of transcription, DNA-templated
phosphorylation
chromatin modification
Performance Guarantee
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