Invitrogen

Phospho-PKR (Thr446) Polyclonal Antibody

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Datasheet
Protocols
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Datasheet
Protocols
Questions & Answers

Product Details

PA5-121356

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000-1:5,000
-

Immunocytochemistry (ICC/IF)

1:50-1:200
-

ELISA (ELISA)

1 µg/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic phosphorylated peptide around T446 of human PKR/EIF2AK2 (NP_002750.1).
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.89 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2914928

Product Specific Information

Positive test controls include: HeLa. The target is usually found in the following locations: Cytoplasm, Nucleus, perinuclear region.

Immunogen sequence: KRTRSK

Target Information

EIF2AK2 (PKR) is one of 4 kinases that specifically phosphorylate Ser51 of translation initiation factor eIF2-alpha in response to various environmental stresses. EIF2AK2 plays a key role in antiviral defense mediated through its direct activation by double-stranded RNA produced by viral infection. PKR plays a key role in IFN induced-innate antiviral response, virus-induced apoptosis, cell growth & differentiation. PKR induces apoptosis by up-regulating Fas expression and mediates FADD/Caspase-8 death signaling pathway. Upon viral infection, it functions as a dual protein, sequentially activating both cell survival & cell death pathways using kinase independent and dependent strategies. PRKR regulates multiple pathways which include NF-kappaB activation, p53, p38, & PDGF signaling pathway. PKR has been implicated in tumor suppression & malignancy. To evade the antiviral effects of PKR, viruses have evolved multiple mechanisms, such as the inhibition of PKR by the non-structural protein (NS1) of the influenza virus. More recently, PKR has been implicated in several neurodegenerative diseases including Alzheimer, Huntington, and amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: double stranded RNA activated protein kinase; dsRNA-activated kinase; EC 2.7.11.1; eIF-2 alpha; eIF-2A protein kinase 2; eIF2a Kinase; eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; IFN- type I-induced and dsRNA-activated kinase; IFN-induced and double-stranded RNA-activated kinase; Interferon-induced, double-stranded RNA-activated protein kinase; interferon-inducible elF2alpha kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; OTTHUMP00000201320; P1/eIF-2A protein kinase; p68 kinase; PKR; protein kinase R; Protein kinase RNA-activated; protein kinase, interferon inducible double stranded RNA dependent; protein kinase, interferon-inducible double stranded RNA dependent; protein phosphatase 1, regulatory subunit 83; Serine/threonine-protein kinase TIK; T-cell viral integration site; Tyrosine-protein kinase EIF2AK2

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Gene Aliases: 2310047A08Rik; 4732414G15Rik; AI467567; AI747578; EIF2AK1; EIF2AK2; PKR; PPP1R83; PRKR; Tik

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UniProt ID: (Human) P19525, (Mouse) Q03963

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Entrez Gene ID: (Human) 5610, (Mouse) 19106

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Function(s)
double-stranded RNA binding protein kinase activity protein serine/threonine kinase activity eukaryotic translation initiation factor 2alpha kinase activity non-membrane spanning protein tyrosine kinase activity protein binding ATP binding protein phosphatase type 2A regulator activity poly(A) RNA binding nucleotide binding RNA binding protein tyrosine kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups non-receptor serine/threonine protein kinase
Process(es)
activation of MAPKK activity positive regulation of cytokine production transcription, DNA-templated translation protein phosphorylation negative regulation of cell proliferation response to mechanical stimulus response to virus response to toxic substance regulation of translational initiation by eIF2 alpha phosphorylation negative regulation of translation peptidyl-tyrosine phosphorylation evasion or tolerance by virus of host immune response endoplasmic reticulum unfolded protein response response to lipopolysaccharide positive regulation of chemokine production positive regulation of stress-activated MAPK cascade response to vitamin E negative regulation of osteoblast proliferation regulation of protein phosphatase type 2A activity cellular response to amino acid starvation response to interferon-alpha positive regulation of apoptotic process negative regulation of apoptotic process response to exogenous dsRNA negative regulation of viral genome replication innate immune response protein autophosphorylation positive regulation of NF-kappaB transcription factor activity defense response to virus regulation of NLRP3 inflammasome complex assembly positive regulation of NIK/NF-kappaB signaling regulation of hematopoietic progenitor cell differentiation regulation of hematopoietic stem cell proliferation regulation of hematopoietic stem cell differentiation immune system process regulation of transcription, DNA-templated phosphorylation
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