|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 777-789 of P19174.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5 ug/test|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, mouse, rat, Xenopus and zebrafish based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Phospholipase C-gamma 1 (PLCG1), one of two isoforms of the PLC-gamma subtype, is a tyrosine kinase substrate that catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-triphosphate and diacylglycerol, which act as intracellular secondary messengers for calcium mobilization and protein kinase C activation. PLCG1 is found in tissues and cell lines, whereas, PLCG2 is expressed mostly in hematopoietic cell types. PLCG1 plays a crucial part of mammalian growth and development, as mice deficient in PLCG1 die soon after embryonic day 9. PLCG1 is also crucial in growth factor-dependent signal transduction by relaying information downstream through the production of the secondary messengers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
||Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.||Zikherman J,Doan K,Parameswaran R,Raschke W,Weiss A||Proceedings of the National Academy of Sciences of the United States of America (109:E3)||2012|
|Mouse||Not Cited||Quantitative differences in CD45 expression unmask functions for CD45 in B-cell development, tolerance, and survival.||Zikherman J,Doan K,Parameswaran R,Raschke W,Weiss A||Proceedings of the National Academy of Sciences of the United States of America (109:E3)||2012|