Western blot analysis of Phospho-PLD2 pTyr169 using Phospho-PLD2 pTyr169 polyclonal antibody (Product # PA5-36868) at a dilution of 1:500. Lane 1: HEK293T cell lysate treated with TNF-alpha (20ng/ml, 15min), Lane 2: NIH-3T3 cell lysate treated with TNF-alpha (20ng/ml, 15min), Lane 3: H9C2 cell lysate treated with TNF-alpha (20ng/ml, 15min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human PLD2 around the phosphorylation site of Tyrosine 169|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 106 kDa.
Purity is >95% by SDS-PAGE.
Phosphatidylcholine phospholipase D1 and D2 (PC-PLD1 and PC-PLD2) are phospholipid-specific phosphodiesterases that hydrolyze phosphatidylcholine. Unlike PC-PLD1, which associates with secretory granules, PC-PLD2 localizes to the plasma membrane, where it is implicated in the formation of endocytotic vesicles. Both PC-PLD1 and PC-PLD2 coordinately regulate macrophage phagocytosis. PC-PLD activity in mammalian cells is transiently stimulated upon activation by G protein-coupled and receptor tyrosine kinase cell surface receptors. In addition, tubulin binding to PC-PLD2 inhibits muscarinic receptor-linked PC-PLD2 activation. PC-PLD2 also enhances PKCzeta activity through direct interaction in a lipase activity-independent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.