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Invitrogen

Phospho-PLK1 (Thr210) Polyclonal Antibody

View all (60) PLK1 antibodies
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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-104633

Applications Tested Dilution Publications

Western Blot (WB)

 1:500-1:2,000 -

Immunohistochemistry (Paraffin) (IHC (P))

 1:50-1:200 -
Product Specifications

Species Reactivity

 Human, Mouse, Rat

Host/Isotype

 Rabbit / IgG

Class

 Polyclonal

Type

 Antibody

Immunogen

A synthesized peptide derived from human PLK1 around the phosphorylation site of Thr210.
View immunogen

Conjugate

 Unconjugated

Form

 Liquid

Concentration

 1 mg/mL

Purification

 sequential chromatography

Storage buffer

 PBS, pH 7.4, with 50% glycerol

Contains

 0.02% sodium azide

Storage conditions

 -20°C

RRID

AB_2816108

Target Information

PLK1 is a Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, p73/TP73, TPT1 and WEE1. Plays a key role in centrosome functions and the assembly of bipolar spindles by phosphorylating KIZ, NEDD1 and NINL.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cell cycle regulated protein kinase; PLK 1; PLK-1; polo (Drosophia)-like kinase; polo like kinase; polo-like kinase; Polo-like kinase 1; polo-like kinase homolog; Serine/threonine-protein kinase 13; Serine/threonine-protein kinase PLK1; STPK13; STPK14

Gene Aliases: PLK; PLK1; STPK13

UniProt ID: (Human) P53350, (Mouse) Q07832, (Rat) Q62673

Entrez Gene ID: (Human) 5347, (Mouse) 18817, (Rat) 25515

Molecular Function: non-receptor serine/threonine protein kinase protein modifying enzyme

Function(s)
mitotic sister chromatid segregation G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter mitotic cytokinesis cytokinesis microtubule bundle formation protein phosphorylation sister chromatid cohesion mitotic nuclear division mitotic nuclear envelope disassembly mitotic spindle assembly checkpoint regulation of mitotic cell cycle cell proliferation positive regulation of peptidyl-threonine phosphorylation female meiosis chromosome segregation protein ubiquitination peptidyl-serine phosphorylation regulation of mitotic metaphase/anaphase transition anaphase-promoting complex-dependent catabolic process G2 DNA damage checkpoint protein destabilization positive regulation of proteasomal ubiquitin-dependent protein catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process negative regulation of apoptotic process regulation of protein binding homologous chromosome segregation establishment of protein localization negative regulation of cyclin-dependent protein serine/threonine kinase activity positive regulation of proteolysis centrosome organization positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein transferase activity regulation of cell cycle synaptonemal complex disassembly protein localization to chromatin positive regulation of protein localization to nucleus regulation of mitotic spindle assembly regulation of cell cycle G2/M phase transition positive regulation of ubiquitin protein ligase activity cell cycle phosphorylation polar body extrusion after meiotic divisions cell division response to antibiotic
Process(es)
protein kinase activity protein serine/threonine kinase activity protein binding ATP binding microtubule binding anaphase-promoting complex binding kinase activity protein kinase binding nucleotide binding transferase activity transferase activity, transferring phosphorus-containing groups
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