Immunofluorescent analysis of Phospho-PLK1 pThr210 (green) in HeLa cells either left untreated (left panel) or treated with 50nM Nocodazole (right panel) for 16 hours. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a Phospho-PLK1 pThr210 polyclonal antibody (Product # PA1-126) at a dilution of 1:400 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin (Product # 21834) and nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ArrayScan and ToxInsight Instrument at 20X magnification.
|Tested species reactivity||Fruit fly, Human, Mouse, Rat|
|Published species reactivity||Fruit fly|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a portion of mouse PLK1 phosphorylated at Thr210.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine, pH 7.0, with 43% glycerol|
|Contains||0.09% sodium azide|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1:100 - 1:1000|
|Western Blot (WB)||1ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
Recognizes human, mouse (weak), rat (weak) and Drosophila (weak).
This antibody was originally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Polo-like Kinase 1 (PLK1) is found to have several functions in mitotic progression. It also has been found to be elevated in a broad range of human tumors. The function of PLK1 appears to be a requirement for proper spindle assembly and other centrosome microtubule events.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Fruit fly||Not Cited||
An Amino-Terminal Polo Kinase Interaction Motif Acts in the Regulation of Centrosome Formation and Reveals a Novel Function for centrosomin (cnn) in Drosophila.
PA1-126 was used in ChIP assay to investigate the formation of the pericentriolar matrix.
|Eisman RC,Phelps MA,Kaufman T||Genetics (201:685)||2015|