Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Western blot analysis of Phospho-PP2A alpha pTyr307 using Phospho-PP2A alpha pTyr307 polyclonal antibody (Product # PA5-36874) at a dilution of 1:500. Lane 1: HEK293T cells treated with EGF (0.1ng/ml, 30min), Lane 2: Mouse brain tissue lysate, Lane 3: H9C2 cells treated with EGF (0.1ng/ml, 30min).
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide derived from human PP2A-alpha around the phosphorylation site of Tyrosine 307|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody detects endogenous protein at a molecular weight of 38 kDa.
Purity is >95% by SDS-PAGE.
In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. The PP2A family comprises subfamily members PP2A alpha and PP2A beta. An additional protein phosphatase catalytic subunit, PPX (also known as PP4) is a putative member of a novel PP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PP2A alpha; PP2A-alpha; PPP2CA; protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isoform; protein phosphatase 2, catalytic subunit, alpha isozyme; protein phosphatase 2a, catalytic subunit, alpha isoform; protein phosphatase-2A-alpha; Replication protein C; RP-C; serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform
PP2A; Pp2a1; PP2Ac; PP2CA; PP2Calpha; PPP2CA; R75353; RP-C