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Phospho-PRK1/PRK2 (Thr774, Thr816) Antibody (PA5-118729) in ICC/IF
Immunocytochemistry analysis of Phospho-PRK1/PRK2 (Thr774, Thr816) in A549 cells (H2O2 treatment). The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with Phospho-PRK1/PRK2 (Thr774, Thr816) polyclonal antibody (Product # PA5-118729) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugate... View More
Product Details
PA5-118729
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PKN1(Accession Q16512), corresponding to amino acid residues around phosphorylated Thr774.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2903229
Product Specific Information
Antibody detects endogenous levels of PRK1/2 only when phosphorylated at Thr774/816.
Target Information
Receptor-like kinase involved in the control of pollen germination and pollen tube polar growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cardiolipin-activated protein kinase Pak2; p140 kinase; PAK-1; PKN gamma; Protease-activated kinase 1; protease-activated kinase 2; Protein kinase C-like 1; Protein kinase C-like 2; Protein kinase C-like PKN; protein kinase C-related kinase 1; protein kinase PKN; Protein kinase PKN-alpha; Protein-kinase C-related kinase 1; Protein-kinase C-related kinase 2; serine-threonine kinase N; Serine-threonine protein kinase N; serine/threonine protein kinase N; Serine/threonine-protein kinase N1; Serine/threonine-protein kinase N2
Gene Aliases: DBK; PAK-1; Pak-2; PAK1; PAK2; PKN; PKN-ALPHA; PKN1; PKN2; PRK1; PRK2; PRKCL1; PRKCL2; PRO2042; STK7
UniProt ID: (Human) Q16512, (Human) Q16513, (Rat) Q63433
Entrez Gene ID: (Human) 5585, (Human) 5586, (Rat) 29355, (Rat) 207122
chromatin binding
protein kinase activity
protein serine/threonine kinase activity
protein kinase C activity
protein kinase C binding
protein binding
ATP binding
GTP-Rho binding
ligand-dependent nuclear receptor transcription coactivator activity
histone kinase activity (H3-T11 specific)
histone binding
histone deacetylase binding
Rac GTPase binding
androgen receptor binding
kinase activity
poly(A) RNA binding
RNA polymerase binding
cadherin binding involved in cell-cell adhesion
non-receptor serine/threonine protein kinase
B cell homeostasis
B cell apoptotic process
regulation of germinal center formation
regulation of immunoglobulin production
renal system process
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein phosphorylation
negative regulation of protein kinase activity
hyperosmotic response
signal transduction
activation of JUN kinase activity
epithelial cell migration
peptidyl-serine phosphorylation
negative regulation of B cell proliferation
histone H3-T11 phosphorylation
spleen development
regulation of cell motility
regulation of transcription, DNA-templated
apoptotic process
cell cycle
positive regulation of cytokinesis
intracellular signal transduction
apical junction assembly
positive regulation of viral genome replication
positive regulation of mitotic cell cycle
cell division
cell-cell adhesion
negative regulation of protein phosphorylation
cell adhesion
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