This Antibody was verified by Cell Treatment to ensure that the antibody binds to the antigen stated. View Details
This product contains enough material for 10 mini-blots. Antibody has been negatively preadsorbed using a nonphosphopeptide then purified by epitope-specific affinity chromatography.
The antibody has been used in western blotting and immunohistochemistry. Other applications may work but have not been tested. Positive controls used were CEF cell extracts +/- Pyk2 transfection or A431 whole cell lysate.
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the MAP kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Pyk2 (proline-rich/Ca2+-activated tyrosine kinase; FAK2) is a member of the FAK subfamily family of non-receptor, proline-rich protein tyrosine kinases and lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. Pyk2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines, and certain stress stimuli (reactive oxygen species and Ca2+ flux). Pyk2 undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. Pyk2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B cell receptor signaling, and cell death. Pyk2 tyrosine 402 is autophosphorylated, an important initial step for full activation of the kinase. This phosphorylation also allows binding of Src and the p85 subunit of PI3-kinase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CADTK; CAK beta; CAK-beta; calcium-dependent tyrosine kinase; calcium-regulated non-receptor proline-rich tyrosine kinase; cell adhesion kinase beta; cellular adhesion kinase beta; FADK 2; FADK2; Focal Adhesion Kinase 2; proline-rich tyrosine kinase 2; protein kinase B; protein tyrosine kinase 2 beta; protein-tyrosine kinase 2-beta; PTK; PTK2 protein tyrosine kinase 2 beta; PTK2B; PTK2B protein tyrosine kinase 2 beta; related adhesion focal tyrosine kinase
Gene Aliases: CADTK; CAKB; CAKbeta; E430023O05Rik; FADK2; FAK2; PKB; PTK; PYK2; RAFTK