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Western blot of Phospho-Parkin pSer101 in HEK293 cells transfected with Parkin WT (Phospho) and Parkin Ser378 mutant (non-phospho) using a polyclonal antibody (Product # PA1-4735). Results show the phospho-specific immunolabeling of the ~ 52k Parkin protein and lack of immunolabeling in the Parkin Ser378 mutant.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser378 of human PARK2 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
The precise function of this gene is unknown; however, the encoded protein is a component of a multiprotein E3 ubiquitin ligase complex that mediates the targeting of substrate proteins for proteasomal degradation. Mutations in this gene are known to cause Parkinson disease and autosomal recessive juvenile Parkinson disease. Alternative splicing of this gene produces multiple transcript variants encoding distinct isoforms. Additional splice variants of this gene have been described but currently lack transcript support.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Diminished parkin solubility and co-localization with intraneuronal amyloid-β are associated with autophagic defects in Alzheimer's disease.
PA1-4735 was used in western blot to study the decreased solubility of the parkin E3 ubiquitin ligase observed in Alzheimer's disease brain and its role in the autophagic clearance of Abeta(1-42)
|Lonskaya I,Shekoyan AR,Hebron ML,Desforges N,Algarzae NK,Moussa CE||Journal of Alzheimer's disease : JAD (33:231)||2012|
AR-JP; LPRS2; Parkinson disease (autosomal recessive, juvenile) 2, parkin; parkinson juvenile disease protein 2; parkinson protein 2 E3 ubiquitin protein ligase; parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PRKN
AR-JP; LPRS2; PARK2; PDJ; PRKN