|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:500|
|Immunocytochemistry (ICC)||See 12 publications below|
|Western Blot (WB)||See 21 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunofluorescence (IF)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat , Pig , Human , Mouse , Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated paxillin, and ii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine, irrespective of the sequence. The final product is generated by affinity chromatography using a paxillin-derived peptide that is phosphorylated at tyrosine 118.
Positive controls used with this antibody in western blotting were normal mouse mammary gland (NMuMG) cells, NMuMG transfected with EGFP-tagged paxillin +/- TGFbeta, and human epidermoid carcinoma cells (A431) +/- EGF.
Paxillin is a 68 kDa cytoskeletal adapter protein and a substrate for several tyrosine kinases such as Src, FAK, and p210BRC/ABL. It is involved in organization and function of focal adhesions, which are critical to cell adhesion and migration. This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation, and cancer. Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins, and other adaptor proteins (e.g., actin, vinculin, Crk). The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Tyrosine 118, a Crk binding site of paxillin, is phosphorylated by FAK or Src during hormone stimulation or integrin-mediated cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
Gene Aliases: AW108311; AW123232; Pax; PXN
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