|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:500|
|Immunocytochemistry (ICC)||See 11 publications below|
|Western Blot (WB)||See 20 publications below|
|Miscellaneous PubMed (MISC)||See 4 publications below|
|Immunofluorescence (IF)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat , Pig , Human , Mouse , Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human paxillin that contains tyrosine 118.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
Paxillin is a focal adhesion protein and a substrate for several tyrosine kinases such as src, FAK, and p210BRC/ABL. The tyrosine phosphorylation of paxillin is affected by conditions that change cell-cell adhesion. Paxillin associates tightly with FAK and Crk through its SH2 domain. This interaction is independent of the extracellular matrix. Although paxillin was initially discovered in fibroblasts, its phosphorylation may also be important during neurite extension during differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: PAX-1; PAXI; Paxillin; paxillin alpha; testicular tissue protein Li 134
Gene Aliases: AW108311; AW123232; Pax; PXN
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