Western blot analysis of phospho Paxillin (Y118) in recombinant fusion protein containing 1) a phosphorylated tyrosine at position 118 and 2) an unphosphorylated tyrosine at position 118, using a Phospho-Paxillin pTyr118 polyclonal antibody (Product # PA5-23327) at 0.025 ug/ml.
|Tested species reactivity||Dog, Chicken, Human, Mouse, Non-human primate, Rat, Zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (111 VGEEEHV(Y)SFPNKQK 125) of Phospho-Paxillin pTyr118 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.025-0.1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Paxillin is a protein with four LIM domains, a proline-rich domain containing a consensus SH3-binding site, and three potential SH2-binding sites. It acts as an ERK-regulated scaffold that coordinates FAK and Rac activation in epithelial morphogenesis. Paxillin is a novel vinculin-binding protein present in focal adhesions and its isoforms in monocyte and cancer cells are known to have different binding properties to focal adhesion proteins. Paxillin binds to schwannomin and regulates its density-dependent localization and effect on cell morphology. Phosphorylation of Paxillin by JNK is known to regulate cell migration. The human Paxillin gene is localized in the chromosomal region 12q24.31.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.