|Tested species reactivity||Bovine, Dog, Human, Mouse, Pig, Rabbit, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH-conjugated, corresponding to residues R(14) A [pS] T I E M P Q Q A R(25) C of human cardiac phospholamban.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.2M tris glycine with 0.15M NaCl, 30% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-25784 detects Phospholamban (phospho S16) from human, canine, mouse, rabbit, rat, porcine and bovine samples. PA1-25784 is expected to cross react with rabbit, mouse, rat, canine, and porcine due to sequence homology.
PA1-25784 has been successfully used in Western blot procedures.
The PA1-25784 immunogen is a synthetic peptide, KLH-conjugated, corresponding to residues R(14) A [pS] T I E M P Q Q A R(25) C of human cardiac phospholamban. The immunizing sequence is conserved in mouse, rat, rabbit, pig and dog.
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The onset of left ventricular diastolic dysfunction in SHR rats is not related to hypertrophy or hypertension.
PA1-25784 was used in western blot to investigate the relationship between left ventricular diastolic dysfunction and abnormal intracellular calcium homeostasis
|Dupont S,Maizel J,Mentaverri R,Chillon JM,Six I,Giummelly P,Brazier M,Choukroun G,Tribouilloy C,Massy ZA,Slama M||American journal of physiology. Heart and circulatory physiology (302:H1524)||2012|