Immunofluorescent analysis of Phosphorylated Progesterone Receptor using Phospho-Progesterone Receptor pSer294 Monoclonal Antibody (608) (Product# MA1-414 ) shows staining in MCF-7 Cells. Phospho-Progesterone Receptor pSer294 (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Phospho-Progesterone Receptor pSer294 (Product# MA1-414 ) at a dilution of 1:20 over night at 4 °C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product# 35552 for GAR, Product# 35503 for GAM). Images were taken at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Rat, Mouse, Human, Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phosphopeptide corresponding to residues P(288) M A P G R S(p) P L A T T V(300) of human Progesterone Receptor.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-414 detects phospho-progesterone receptor (pSer294) from human samples.
MA1-414 has been successfully used in Western blot, ELISA, immunohistochemistry, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer294) A and B from T47D cell extract.
The MA1-414 immunogen is a synthetic peptide corresponding to residues P(288) M A P G R S(p) P L A T T V(300) of human Progesterone Receptor
The progesterone receptor is a member of the Steroid Hormone Receptor Superfamily. It exists in two distinct isoforms in human cells. PR-B is the transcriptionally active form and is responsible for activating genes for the maintainence of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. Phospholabeling studies have shown that PR is phosphorylated in at least 9 different locations in response to progestins, which suggests that PR affects many different cellular pathways.
Ser 294 is one of four sites that demonstrate basal phosphorylation in the absence of ligand. The level of phosphorylation rapidly increases with addition of receptor agonists. This antibody recognizes the phosphorylated form of both PR-A and PR-B at Ser 294.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
A Common Docking Domain in Progesterone Receptor-B links DUSP6 and CK2 signaling to proliferative transcriptional programs in breast cancer cells.
MA1-414 was used in western blot to characterize the N-terminal common docking domain of the progesterone receptor
|Hagan CR,Knutson TP,Lange CA||Nucleic acids research (41:8926)||2013|
Protein kinases mediate ligand-independent derepression of sumoylated progesterone receptors in breast cancer cells.
MA1-414 was used in western blot to investigate the mechanism of progesterone receptor sumoylation in breast cancer cells
|Daniel AR,Lange CA||Proceedings of the National Academy of Sciences of the United States of America (106:14287)||2009|
Activation of Stat3 by heregulin/ErbB-2 through the co-option of progesterone receptor signaling drives breast cancer growth.
MA1-414 was used in western blot to investigate the role of Stat3 in the tumorigenic effect of heregulin/ErbB-2 in breast cells
|Proietti CJ,Rosemblit C,Beguelin W,Rivas MA,Díaz Flaqué MC,Charreau EH,Schillaci R,Elizalde PV||Molecular and cellular biology (29:1249)||2009|
Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis.
MA1-414 was used in western blot to study the role of cancer-associated fibroblasts in mammary tumorigenesis and development of hormone independence
|Giulianelli S,Cerliani JP,Lamb CA,Fabris VT,Bottino MC,Gorostiaga MA,Novaro V,Góngora A,Baldi A,Molinolo A,Lanari C||International journal of cancer (123:2518)||2008|
Linkage of progestin and epidermal growth factor signaling: phosphorylation of progesterone receptors mediates transcriptional hypersensitivity and increased ligand-independent breast cancer cell growth.
MA1-414 was used in western blot to study the effects of EGF on progesterone receptor function and regulation of breast cancer cell growth
|Daniel AR,Qiu M,Faivre EJ,Ostrander JH,Skildum A,Lange CA||Steroids (72:188)||2007|
Progesterone receptors induce cell cycle progression via activation of mitogen-activated protein kinases.
MA1-414 was used in western blot to study the mechanism by which progesterone receptors induce cell cycle progression
|Skildum A,Faivre E,Lange CA||Molecular endocrinology (Baltimore, Md.) (19:327)||2005|
Inhibition of in vivo breast cancer growth by antisense oligodeoxynucleotides to type I insulin-like growth factor receptor mRNA involves inactivation of ErbBs, PI-3K/Akt and p42/p44 MAPK signaling pathways but not modulation of progesterone receptor activity.
MA1-414 was used in western blot to study the mechanism by which antisense oligodeoxynucleotides to type I IGF receptor mRNA inhibit in vivo breast cancer growth
|Salatino M,Schillaci R,Proietti CJ,Carnevale R,Frahm I,Molinolo AA,Iribarren A,Charreau EH,Elizalde PV||Oncogene (23:5161)||2004|
Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects.
MA1-414 was used in immunocytochemistry to study the effect ofprogestins and RU-486 on murine mammary carcinoma
|Bottino MC,Cerliani JP,Rojas P,Giulianelli S,Soldati R,Mondillo C,Gorostiaga MA,Pignataro OP,Calvo JC,Gutkind JS,Molinolo AA,Lüthy IA,Lanari C||Breast cancer research and treatment (126:621)||2011|
Immunoneutralization of inhibin in cycling rats increases follicle-stimulating hormone secretion, stimulates the ovary and attenuates progesterone receptor-dependent preovulatory luteinizing hormone secretion.
MA1-414 was used in immunohistochemistry to investigate the effect of inhibin blockade on sex hormone production in rats
|Gordon A,Aguilar R,Garrido-Gracia JC,Guil-Luna S,Sánchez Cespedes R,Millán Y,Watanabe G,Taya K,Martín de Las Mulas J,Sánchez-Criado JE||Neuroendocrinology (91:291)||2010|
Mitogen-activated protein kinase regulates nuclear association of human progesterone receptors.
MA1-414 was used in immunohistochemistry to investigate the role of mitogen-activated protein kinase during nuclear association of human progesterone receptors
|Qiu M,Olsen A,Faivre E,Horwitz KB,Lange CA||Molecular endocrinology (Baltimore, Md.) (17:628)||2003|