Invitrogen

Phospho-Progesterone Receptor (Ser190) Polyclonal Antibody

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Phospho-Progesterone Receptor (Ser190) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-Progesterone Receptor (Ser190) in mouse kidney tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-Progesterone Receptor (Ser190) polyclonal antibody (Product # PA5-104635) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-Progesterone Receptor (Ser190) Antibody in Western Blot (WB) — Western blot analysis of Phospho-Progesterone Receptor (Ser190) in T47D cells treated with promegestone (Lane 1: treated with phospho-blocking peptide; Lane 2: treated with non-phospho-blocking peptide). Samples were incubated with Phospho-Progesterone Receptor (Ser190) polyclonal antibody (Product # PA5-104635).

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Western Blot (WB)

1:500-1:2000

-

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human Progesterone Receptor around the phosphorylation site of Ser190.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol, 150mM NaCl

Contains

0.02% sodium azide

Storage conditions

-20°C

RRID

AB_2816110

Target Information

The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Nuclear receptor subfamily 3 group C member 3; PR; Progesterone receptor

Gene Aliases: 9930019P03Rik; BB114106; ENSMUSG00000074510; NR3C3; PGR; PR; PR-A; PR-B

UniProt ID: (Human) P06401, (Mouse) Q00175

Entrez Gene ID: (Human) 5241, (Mouse) 18667

Molecular Function: nuclear hormone receptor nucleic acid binding receptor transcription factor

transcription initiation from RNA polymerase II promoter signal transduction cell-cell signaling negative regulation of gene expression steroid hormone mediated signaling pathway positive regulation of transcription from RNA polymerase II promoter ovulation from ovarian follicle epithelial cell maturation transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of cell proliferation positive regulation of smooth muscle cell migration intracellular receptor signaling pathway mammary gland development paracrine signaling response to cocaine negative regulation of apoptotic process positive regulation of transcription, DNA-templated lung alveolus development regulation of epithelial cell proliferation progesterone receptor signaling pathway female mating behavior tertiary branching involved in mammary gland duct morphogenesis

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding steroid hormone receptor activity steroid binding protein binding zinc ion binding enzyme binding ATPase binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding receptor binding lipid binding hormone binding sequence-specific DNA binding metal ion binding