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Immunofluorescent analysis of Phospho-Progesterone Receptor pSer190 in untreated T47D cells (left panel) or stimulated cells with 100 nM of Progesterone (right panel). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospho-Progesterone Receptor pSer190 Monclonal Antibody (1154) (Product # MA1-413) at a dilution of 1:50 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was stained with a fluorescent phalloidin and nuclei (blue) were stained with DAPI. Images were taken at a 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic phosphopeptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 ug/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||4 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-413 detects phospho-progesterone receptor (pSer190) from human samples.
MA1-413 has been successfully used in Western blot, immunohistochemistry, ELISA, immunofluorescence, and immunoprecipitation procedures. By Western blot, this antibody detects ~81 and ~116 kDa proteins representing phospho-progesterone receptor (pSer190) A and B from T47D cell and human breast carcinoma extracts. Immunohistochemical staining of phospho-progesterone receptor (pSer190) (paraffin samples) can be performed using MA1-413.
The MA1-413 immunogen is a synthetic peptide corresponding to residues V(184) L P R G L S(p) P A R Q L L(196) of human Progesterone Receptor.
The progesterone receptor is a member of the Steroid Hormone Receptor Superfamily. It exists in two distinct isoforms in human cells. PR-B is the transcriptionally active form and is responsible for activating genes for the maintainence of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. Phospholabeling studies have shown that PR is phosphorylated in at least 9 different locations in response to progestins, which suggests that PR affects many different cellular pathways.
Ser 190 is one of four sites that demonstrate basal phosphorylation in the absence of ligand. The level of phosphorylation rapidly increases with addition of receptor agonists. This antibody recognizes the phosphorylated form of both PR-A and PR-B at Ser 190.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Classical membrane progesterone receptors in murine mammary carcinomas: agonistic effects of progestins and RU-486 mediating rapid non-genomic effects.
MA1-413 was used in immunocytochemistry to study the effect ofprogestins and RU-486 on murine mammary carcinoma
|Bottino MC,Cerliani JP,Rojas P,Giulianelli S,Soldati R,Mondillo C,Gorostiaga MA,Pignataro OP,Calvo JC,Gutkind JS,Molinolo AA,Lüthy IA,Lanari C||Breast cancer research and treatment (126:621)||2011|
Mutational analysis of progesterone receptor functional domains in stable cell lines delineates sets of genes regulated by different mechanisms.
MA1-413 was used in western blot to study the role of progesterone receptor functional domains in different modes of gene regulation using mutational analysis
|Quiles I,Millán-Ariño L,Subtil-Rodríguez A,Miñana B,Spinedi N,Ballaré C,Beato M,Jordan A||Molecular endocrinology (Baltimore, Md.) (23:809)||2009|
Carcinoma-associated fibroblasts activate progesterone receptors and induce hormone independent mammary tumor growth: A role for the FGF-2/FGFR-2 axis.
MA1-413 was used in western blot to study the role of cancer-associated fibroblasts in mammary tumorigenesis and development of hormone independence
|Giulianelli S,Cerliani JP,Lamb CA,Fabris VT,Bottino MC,Gorostiaga MA,Novaro V,Góngora A,Baldi A,Molinolo A,Lanari C||International journal of cancer (123:2518)||2008|
NR3C3; nuclear receptor subfamily 3 group C member 3; PGR
NR3C3; PGR; PR