|Tested species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (638 SASELPA(S)QPQPFS 651) of Phospho-RAD17 pSer645 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.025-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Rad17, also known as 'cell cycle checkpoint protein RAD17,' is a nuclear protein belonging to the Rad17/RAD24 family and necessary for sustained cell growth, maintenance of chromosomal stability, and ATR-dependent checkpoint activation upon DNA damage. Rad17 is known to have a weak ATPase activity and is required for binding to chromatin and also participates in the recruitment of the RAD1-RAD9-HUS1 complex onto chromatin, and in CHEK1 activation. It may also serve as a sensor of DNA replication progression, and may be involved in homologous recombination. Rad17 is a part of a DNA-binding complex containing RFC2, RFC3, RFC4 and RFC5. Four isoforms have been reported by X-ray irradiation. Overexpression of this protein is seen in various cancer cell lines and in colon carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.