|Tested species reactivity||Human|
|Published species reactivity||Mouse, Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Rb that contains threonine 821 (based on Swiss Protein database, accession number P06400). The sequence is conserved in mouse and rat.|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.3, with 50% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Rb is a tumor suppressor nuclear phosphoprotein capable of binding to DNA. It is phosphorylated on serine and threonine, but not on tyrosine residues. It forms a complex with SV40 large T antigen, adenovirus E1A, and human papilloma virus-16 E. Rb protein may act by regulating transcription and loss of its function leads to uncontrolled cell growth. Aberrations in the RB gene have been implicated in cancers of breast, colon, prostate, kidney, nasopharynx, and leukemia. Phosphorylation of Rb at S608 depends on CDK4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Poldip2 knockout results in perinatal lethality, reduced cellular growth and increased autophagy of mouse embryonic fibroblasts.
44-582G was used in western blot to demonstrate a novel role for Poldip2 in regulating the cell cycle
|Brown DI,Lassègue B,Lee M,Zafari R,Long JS,Saavedra HI,Griendling KK||PloS one (9:null)||2014|
|Human||Not Cited||Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells.||Kollár P,Bárta T,Závalová V,Smejkal K,Hampl A||British journal of pharmacology (162:1534)||2011|
|Human||Not Cited||Cyclin-dependent kinase-like function is shared by the beta- and gamma- subset of the conserved herpesvirus protein kinases.||Kuny CV,Chinchilla K,Culbertson MR,Kalejta RF||PLoS pathogens (6:null)||2010|
|Human||Not Cited||Dual targeting of CDK and tropomyosin receptor kinase families by the oral inhibitor PHA-848125, an agent with broad-spectrum antitumor efficacy.||Albanese C,Alzani R,Amboldi N,Avanzi N,Ballinari D,Brasca MG,Festuccia C,Fiorentini F,Locatelli G,Pastori W,Patton V,Roletto F,Colotta F,Galvani A,Isacchi A,Moll J,Pesenti E,Mercurio C,Ciomei M||Molecular cancer therapeutics (9:2243)||2010|
|Human||Not Cited||The kinase inhibitor O6-cyclohexylmethylguanine (NU2058) potentiates the cytotoxicity of cisplatin by mechanisms that are independent of its effect upon CDK2.||Harrison LR,Ottley CJ,Pearson DG,Roche C,Wedge SR,Dolan ME,Newell DR,Tilby MJ||Biochemical pharmacology (77:1586)||2009|
|Human||Not Cited||Acetylation by GCN5 regulates CDC6 phosphorylation in the S phase of the cell cycle.||Paolinelli R,Mendoza-Maldonado R,Cereseto A,Giacca M||Nature structural and molecular biology (16:412)||2009|
|Not Applicable||Not Cited||
Downregulation of Fer induces PP1 activation and cell-cycle arrest in malignant cells.
44-582G was used in western blot to assess induction of PP1 activation and cell-cycle arrest in malignant cells by downregulation of Fer
|Pasder O,Shpungin S,Salem Y,Makovsky A,Vilchick S,Michaeli S,Malovani H,Nir U||Oncogene (25:4194)||2006|
|Mouse||Not Cited||Combined loss of Cdk2 and Cdk4 results in embryonic lethality and Rb hypophosphorylation.||Berthet C,Klarmann KD,Hilton MB,Suh HC,Keller JR,Kiyokawa H,Kaldis P||Developmental cell (10:563)||2006|
|Human||Not Cited||Catalytic activities of G1 cyclin-dependent kinases and phosphorylation of retinoblastoma protein in mobilized peripheral blood CD34+ hematopoietic progenitor cells.||Leibundgut K,Schmitz NM,Hirt A||Stem cells (Dayton, Ohio) (23:1002)||2005|
|Human||Not Cited||ICI182,780 induces p21Waf1 gene transcription through releasing histone deacetylase 1 and estrogen receptor alpha from Sp1 sites to induce cell cycle arrest in MCF-7 breast cancer cell line.||Varshochi R,Halim F,Sunters A,Alao JP,Madureira PA,Hart SM,Ali S,Vigushin DM,Coombes RC,Lam EW||The Journal of biological chemistry (280:3185)||2005|
||ICI182,780 induces p21Waf1 gene transcription through releasing histone deacetylase 1 and estrogen receptor alpha from Sp1 sites to induce cell cycle arrest in MCF-7 breast cancer cell line.||Varshochi R,Halim F,Sunters A,Alao JP,Madureira PA,Hart SM,Ali S,Vigushin DM,Coombes RC,Lam EW||The Journal of biological chemistry (280:3185)||2005|
Oxidative stress induces protein phosphatase 2A-dependent dephosphorylation of the pocket proteins pRb, p107, and p130.
44-582G was used in western blot to investigate the roles of pRb, p107, and p130 in the cellular response to oxidative stress.
|Cicchillitti L,Fasanaro P,Biglioli P,Capogrossi MC,Martelli F||The Journal of biological chemistry (278:19509)||2003|