Active Motif

Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (3E8)

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Phospho-RNA pol II CTD (Ser5) Antibody in ChIP assay (ChIP) — RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP. Chromatin immunoprecipitation (ChIP) was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with 10 µg of chromatin from human myeloma LP1 cells and 10 µg RNA pol II CTD phospho Ser5 antibody. ChIP DNA was used in qPCR with the control primer pairs or gene-specific primer pairs as indicated. Data are presented as Binding Events Detected per 1000 Cells using Active Motifs Epigenetic Services normalization scheme which accounts for primer efficiency and the amount of chromatin used in the ChIP reaction.

Phospho-RNA pol II CTD (Ser5) Antibody in ChIP-sequencing (ChIP-Seq) — RNA pol II CTD phospho Ser5 antibody (mAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT® High Sensitivity Kit (Cat. No. 53040) with chromatin from 2.3 million HL60 cells and 5 ug of antibody. ChIP DNA was sequenced on the Illumina HiSeq and 23 million sequence tags were mapped to identify RNA pol II phospho Ser5 binding. Data is compared to ChIP-Seq data using a phospho Ser2 antibody (61083). ChIP-Seq data from three specific genes is shown as an example. The Pol II phospho Ser5 antibody detects polymerase more at the 5180 end of the genes and the phospho Ser2 antibody detects Pol II more toward the 3180 end of the genes.

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

5-10 µg

View 3 publications 3 publications

Immunocytochemistry (ICC)

1:500

Immunofluorescence (IF)

1:500

Western Blot (WB)

0.5-2 µg/mL

ChIP-sequencing (ChIP-Seq)

5-10 µg

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Immunoprecipitation (IP)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Rat, Yeast

Host / Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3E8

Immunogen

This RNA pol II CTD phospho Ser5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

70mM tris, pH 8, with 30% glycerol, 31mM glycine, 105mM NaCl, 0.07mM EDTA

Contains

0.035% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2687451

Target Information

RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (DNA directed), alpha 1, catalytic subunit; polymerase (DNA) alpha 1, catalytic subunit; polymerase (DNA-directed), alpha (70kD); polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8

Gene Aliases: hRPB220; hsRPB1; NSX; p180; POLA; POLA1; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2

UniProt ID: (Human) P09884, (Human) P24928

Entrez Gene ID: (Human) 5422, (Human) 5430

Molecular Function: DNA-directed RNA polymerase RNA binding protein nucleic acid binding nucleotidyltransferase transferase