Active Motif
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
0.5-2 µg/mL | - |
Immunocytochemistry (ICC/IF) |
1:500 | - |
Immunoprecipitation (IP) |
- | View 1 publication 1 publication |
ChIP assay (ChIP) |
5-10 µg | View 3 publications 3 publications |
ChIP-sequencing (ChIP-Seq) |
5-10 µg | View 2 publications 2 publications |
Miscellaneous PubMed (Misc) |
- | View 4 publications 4 publications |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Published species |
Human, Rat, Yeast |
Host/Isotype |
Rat / IgG2a |
Class |
Monoclonal |
Type |
Antibody |
Clone |
3E8 |
Immunogen |
This RNA pol II CTD phospho Ser5 antibody was raised against a peptide containing the RNA pol II CTD sequence phosphorylated at serine 5. |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1 mg/mL |
Purification |
Protein G |
Storage buffer |
70mM tris, pH 8, with 30% glycerol, 31mM glycine, 105mM NaCl, 0.07mM EDTA |
Contains |
0.035% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2687451 |
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8
Gene Aliases: hRPB220; hsRPB1; POLR2; POLR2A; POLRA; RPB1; RPBh1; RpIILS; RPO2; RPOL2
UniProt ID: (Human) P24928
Entrez Gene ID: (Human) 5430
Molecular Function:
DNA-directed RNA polymerase
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