Invitrogen

Phospho-RRN3 (Ser649) Polyclonal Antibody

View all (10) RRN3 antibodies

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Phospho-RRN3 (Ser649) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of paraffin-embedded Phospho-RRN3 (Ser649) in mouse lung tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-RRN3 (Ser649) polyclonal antibody (Product # PA5-103273) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.

Phospho-RRN3 (Ser649) Antibody in Western Blot (WB) — Western blot analysis of Phospho-RRN3 (Ser649) in Jurkat cell lysate. Samples were incubated with Phospho-RRN3 (Ser649) polyclonal antibody (Product # PA5-103273).

Product Details

PA5-103273

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human TIF-IA around the phosphorylation site of Ser649. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	sequential chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C
RRID	AB_2815972

Target Information

Required for efficient transcription initiation by RNA polymerase I. Required for the formation of the competent preinitiation complex (PIC). Dissociates from pol I as a consequence of transcription. In vitro, cannot activate transcription in a subsequent transcription reaction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: RNA polymerase I-specific transcription initiation factor RRN3; RRN3 RNA polymerase I transcription factor homolog; TIF-IA; Transcription initiation factor IA; transcription initiation factor TIF-IA

Gene Aliases: A-270G1.2; AL023001; E130302O19Rik; R75565; RGD1305001; RRN3; TIF-1A; Tif1a; TIFIA

UniProt ID: (Human) Q9NYV6, (Mouse) B2RS91

Entrez Gene ID: (Human) 54700, (Rat) 304714, (Mouse) 106298

Molecular Function: general transcription factor