A suggested positive control for Western blot is Hela cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is Hela cells.
The p90 ribosomal S6 kinases (RSKs) comprise a family of serine/threonine kinases that lie at the terminus of the ERK pathway. In humans, the RSK family consists of four isoforms (RSK1 to-4). RSK family members are unusual among serine/threonine kinases in that they contain two distinct kinase domains, both of which are catalytically functional. Theses kinase dimains are activated in a sequential manner by a series of phosphorylations. RSK regulates gene expression via association and phosphorylation of transcriptional regulators including c-Fos, estrogen receptor, NFkappaB/IkappaB alpha, cAMP-response element-binding protein (CREB). ERK activates the C-terminal kinase of RSK, leading to activation of the N-terminal kinase. Members of the RSK family are present in the cytoplasm as well as the nucleus. Addition of growth factor to the cells results in the activation of both cytosolic and nuclear RSK and the translocation of the cytosolic RSK into the nucleus upon activation. The activation and nuclear translocation of RSK result in phosphorylation and activation of transcription factors.
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Protein Aliases: 90 kDa ribosomal protein S6 kinase 1; 90kD; dJ590P13.1 (ribosomal protein S6 kinase; dJ590P13.1 (ribosomal protein S6 kinase, 90kD, polypeptide 1); HU-1; MAP kinase-activated protein kinase 1a; MAPK-activated protein kinase 1a; MAPKAP kinase 1a; MAPKAPK-1a; MAPKAPK1A; p90-RSK 1; p90RSK1; p90S6K; polypeptide 1); pp90RSK1; ribosomal protein S6 kinase alpha 1; Ribosomal protein S6 kinase alpha-1; ribosomal protein S6 kinase polypeptide 1; ribosomal protein S6 kinase, 90kD, polypeptide 1; Ribosomal protein S6 kinase, 90kD, 1; ribosomal protein S6 kinase, 90kDa, polypeptide 1; Ribosomal S6 kinase 1; RSK; RSK-1; RSK1; S6 protein kinase (Rsk-1); S6K-alpha 1; S6K-alpha-1
Gene Aliases: HU-1; Mapkapk-1a; MAPKAPK1A; p90-Rsk1; p90Rsk; p90Rsk1; p90S6K; RPS6KA1; RSK; Rsk-1; RSK1; S6K-alpha-1
Molecular Function: annexin calcium-binding protein calmodulin intracellular calcium-sensing protein kinase non-receptor serine/threonine protein kinase protein kinase transfer/carrier protein transferase