|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic phosphopeptide corresponding to a portion of human RSK1 containing serine 221.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.3, with 1mg/ml BSA, 50% glycerol|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-24769 detects RSK1 p90 (phospho S221) from human samples. PA1-24769 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology. Cross reactivity with RSK2 is expected (100% conserved). Cross reactivity with RSK3 (80% homology) and RSK4 (90% homology) is possible but has not been tested. Not yet tested in other species.
PA1-24769 has been successfully used in Western blot procedures. A431 and NIH3T3 cells treated with PMA can be used as a positive control for this antibody.
The PA1-24769 immunogen is a synthetic phosphopeptide corresponding to a portion of human RSK1 containing serine 221.
Store at 4C short term. For extended storage, aliquot and store at -20C or below, avoiding freeze-thaw cycles.
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.