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|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids around Ser249 of human AML1.|
|Storage buffer||0.01M HEPES, pH 7.5, with 100µg/ml BSA, 50% glycerol, 0.15M NaCl|
|Tested Applications||Dilution *|
|Flow Cytometry (FACS)||1:50|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:1,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
AML1/Runx1 binds DNA as a monomer and through the Runt domain. DNA binding is increased by heterodimerization with CBFB. Isoform AML1L can neither bind DNA nor heterodimerize and interferes with the transactivation activity of AML1/Runx1. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T cell receptor enhancers, LCK, IL3 and GMCSF promoters. The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. AML1/Runx1 is expressed in all tissues examined except brain and heart, and is expressed at the highest levels in thymus, bone marrow and peripheral blood. Defects in AML1/Runx1 are the cause of familial platelet disorder with associated myeloid malignancy, an autosomal dominant disease characterized by qualitative and quantitative platelet defects, and propensity to develop acute myelogenous leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
acute myeloid leukemia 1 protein; AML1-EVI-1 fusion protein; CBF-alpha-2; core-binding factor, runt domain, alpha subunit 2; oncogene AML-1; PEA2-alpha B; PEBP2-alpha B; polyomavirus enhancer-binding protein 2 alpha B subunit; runt-related transcription factor 1; SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit
AML1; AML1-EVI-1; AMLCR1; CBFA2; EVI-1; PEBP2aB
Nucleoside, nucleotide and nucleic acid metabolism mRNA transcription mRNA transcription regulation Signal transduction Oncogenesis Oncogene Developmental processes Embryogenesis Ectoderm development Neurogenesis Mesoderm development Skeletal development Hematopoiesis Sex determination Cell proliferation and differentiation