|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rat / IgG1|
|Immunogen||A synthetic peptide corresponding to aa GQRDSSYYWEIEAS|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
This antibody does not bind to unactivated Raf-1 or Raf-1 phosphorylated at Y341 and S339. IHC staining requires heat-mediated antigen retrieval using a 10mM citrate buffer for 10-20 min, pH 6.0, followed by cooling at room temperature for 10-20 min.
Raf1 is an important mediator of signal transduction pathway. It is activated through activation by Src and protein kinase C. It further phosphorylates Mek1 and cdc25. It can bind to GTP-bound Ras through N-terminal domain. It also interacts with hsp90 and 14.3.3 family of proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-Raf proto-oncogene, serine/threonine kinase; cRaf; Oncogene RAF1; Proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; v-raf-1 murine leukemia viral oncogene homolog 1; v-raf-1 murine leukemia viral oncogene-like protein 1
Gene Aliases: c-Raf; CMD1NN; CRAF; NS5; RAF; Raf-1; RAF1
UniProt ID: (Human) P04049
Entrez Gene ID: (Human) 5894
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