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|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from human C-RAF around the phosphorylation site of Serine 338|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 50% glycerol|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Concentration is lot-specific and will vary from 0.5-0.6 mg/ml
The Raf family of serine/threonine-specific kinases is comprised of three members (A-Raf, B-Raf, and c-Raf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen-activated protein kinase (MAPK) pathway. c-Raf kinase (also known as Raf-1) is a key 74 kDa signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Phosphorylation of serine 338 creates a recognition site for tyrosine kinases and is critical for the activation of c-Raf. Serine 338 to tyrosine 341 (SSYY) is the phosphoregulatory site which regulates the biological function of the c-Raf tyrosine kinase by oncogenic Ras and Src.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
C-RAF; CRAF; Craf1 transforming; EC 18.104.22.168; kinase Raf1; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; Oncogene RAF1; Protein kinase raf 1; proto-oncogene c-RAF; raf proto-oncogene serine/threonine protein kinase; RAF proto-oncogene serine/threonine-protein kinase; raf-1; RAF1; sarcoma 3611 oncogene; Similar to murine leukemia viral (V-raf-1) oncogene homolog 1; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1
6430402F14Rik; AA990557; BB129353; c-Raf; CRAF; Craf1; D830050J10Rik; NS5; Raf-1; v-Raf
Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Intracellular signaling cascade MAPKKK cascade Oncogenesis Oncogene Developmental processes Gametogenesis Oogenesis Spermatogenesis and motility Embryogenesis Anterior/posterior patterning Segment specification Cell cycle Cell cycle control Apoptosis Cell proliferation and differentiation