Western blot analysis of Phospho-Rb pSer249/pThr252 in whole cell extracts of COLO 205 cells (lanes 1 and 2) using a Phospho-Rb pSer249/pThr252 recombinant rabbit oligoclonal antibody (Product # 710090) at a dilution of 2.5ug/ml. To confirm specificity, competition was performed by preincubation with the phosphopeptide to inhibit antibody binding (lane 2). Results show a band at ~110kDa.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 245–255 of human Rb protein|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-5 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse, rat and non-human primate based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale and purified with Protein A.
ABfinity™ oligoclonal antibodies comprise a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds—the sensitivity of a polyclonal antibody with the specificity of a monoclonal, all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody—recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets when compared with monoclonal antibodies—an oligoclonal antibody has a known mixture of light and heavy chains. This exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Rb, also known as Retinoblastoma, is a tumor suppressor protein that keeps uncontrolled growth and division in check. Rb belongs to the Pocket Protein family whose members have a pocket for binding proteins. It is nuclear protein which is widely expressed in retinal cells. It is inactivated by phosphorylation and activated by dephosphorylation. Phosphorylation of serine 249 and threonine 252 is catalyzed by Cyclin D-cdk4. In humans, the protein is encoded by the RB1 gene located on the q arm of chromosome 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.